Abnormal DAuer Formation Family Member (Daf-2) (C-Term) antibody

Antigen: Abnormal DAuer Formation Family Member (Daf-2)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Caenorhabditis elegans (C. elegans)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN965970

Additional Information

Alternative name: Daf-2

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-terminal residues of Caenorhabditis elegans daf-2(Abnormal dauer formation protein 2)

Description: The daf-2 is an insulin receptor-like gene that regulates longevity and diapause in Caenorhabditis elegans. A C. elegans neurosecretory signaling system regulates whether animals enter the reproductive life cycle or arrest development at the long-lived dauer diapause stage. daf-2, a key gene in the genetic pathway that mediates this endocrine signaling, encodes an insulin receptor family member. Decreases in DAF-2 signaling induce metabolic and developmental changes, as in mammalian metabolic control by the insulin receptor. Decreased DAF-2 signaling also causes an increase in life-span. Lifespan regulation by insulin-like metabolic control is analogous to mammalian longevity enhancement induced by caloric restriction, suggesting a general link between metabolism, diapause, and longevity.

Application Details

Application Notes: ELISA, Western blotting: 1µg/ml for 2hrs.

Purification: Purified by antigen-specific affinity chromatography.

Buffer: This antibody is stored in PBS, 50% glycerol

Preservative: 0.01% Sodium Azide

Storage: Store at -20°C.

Restrictions: For Research Use only

Publications

Product: Kimura, Tissenbaum, Liu et al.: "daf-2, an insulin receptor-like gene that regulates longevity and diapause in Caenorhabditis elegans." in: Science (New York, N.Y.), Vol. 277, Issue 5328, pp. 942-6, 1997 (PubMed).

Ogg, Paradis, Gottlieb et al.: "The Fork head transcription factor DAF-16 transduces insulin-like metabolic and longevity signals in C. elegans." in: Nature, Vol. 389, Issue 6654, pp. 994-9, 1997 (PubMed).

Tissenbaum, Ruvkun: "An insulin-like signaling pathway affects both longevity and reproduction in Caenorhabditis elegans." in: Genetics, Vol. 148, Issue 2, pp. 703-17, 1998 (PubMed).

Yu, Larsen: "DAF-16-dependent and independent expression targets of DAF-2 insulin receptor-like pathway in Caenorhabditis elegans include FKBPs." in: Journal of molecular biology, Vol. 314, Issue 5, pp. 1017-28, 2001 (PubMed).