ER Degradation Enhancer, Mannosidase alpha-Like 1 (EDEM1) (C-Term) antibody

Antigen: ER Degradation Enhancer, Mannosidase alpha-Like 1 (EDEM1)

Synonyms: EDEM, FLJ51559, FLJ51560, KIAA0212, mKIAA0212, A130059K23Rik, RGD1563633, MGC55816, zgc:55816, edem, MGC76118, EDEM1, MGC165902

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966027

Additional Information

Alternative name: EDEM1

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-terminal residues of human EDEM1(ER degradation-enhancing alpha-mannosidase-like 1)

Description: EDEM1(ER degradation-enhancing alpha-mannosidase-like 1) extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-dependent manner. It lacks mannosidase activity. EDEM1 is located in endoplasmic reticulum membrane and is a single-pass type II membrane protein. EDEM1 belongs to the glycosyl hydrolase 47 family.

Application Details

Application Notes: ELISA, Western blotting: 1µg/ml for 2hrs.

Purification: Purified by antigen-specific affinity chromatography.

Buffer: This antibody is stored in PBS, 50% glycerol

Preservative: 0.01% Sodium Azide

Storage: Store at -20°C.

Research Area: Signaling

Restrictions: For Research Use only

Publications

Product: Nagase, Seki, Ishikawa et al.: "Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 3, Issue 5, pp. 321-9, 341-54, 1997 (PubMed).

Molinari, Calanca, Galli et al.: "Role of EDEM in the release of misfolded glycoproteins from the calnexin cycle." in: Science (New York, N.Y.), Vol. 299, Issue 5611, pp. 1397-400, 2003 (PubMed).