GATA1 antibody

Catalog No. ABIN966189

Product Details anti-GATA1 Antibody

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Specificity: GATA-1 (Ab-142) antibody detects endogenous levels of total GATA-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human GATA-1 around the phosphorylation site of serine 142 (R-L-SP-P-D).

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-GATA1 antibody (ABIN966189)

Freson, Devriendt, Matthijs, Van Hoof, De Vos, Thys, Minner, Hoylaerts, Vermylen, Van Geet: "Platelet characteristics in patients with X-linked macrothrombocytopenia because of a novel GATA1 mutation." in: Blood, Vol. 98, Issue 1, pp. 85-92, (2001) (PubMed).

Mehaffey, Newton, Gandhi, Crossley, Drachman: "X-linked thrombocytopenia caused by a novel mutation of GATA-1." in: Blood, Vol. 98, Issue 9, pp. 2681-8, (2001) (PubMed).

Nichols, Crispino, Poncz, White, Orkin, Maris, Weiss: "Familial dyserythropoietic anaemia and thrombocytopenia due to an inherited mutation in GATA1." in: Nature genetics, Vol. 24, Issue 3, pp. 266-70, (2000) (PubMed).

Zon, Tsai, Burgess, Matsudaira, Bruns, Orkin: "The major human erythroid DNA-binding protein (GF-1): primary sequence and localization of the gene to the X chromosome." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 2, pp. 668-72, (1990) (PubMed).

Trainor, Evans, Felsenfeld, Boguski: "Structure and evolution of a human erythroid transcription factor." in: Nature, Vol. 343, Issue 6253, pp. 92-6, (1990) (PubMed).

Target Details for GATA1

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Alternative Name: GATA1 (GATA1 Products)

Pathways: Cellular Response to Molecule of Bacterial Origin