GATA-1 (Ab-142) antibody detects endogenous levels of total GATA-1 protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human GATA-1 around the phosphorylation site of serine 142 (R-L-SP-P-D).
GATA1
Reactivity: Human
WB, ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:500~1:1000 IHC: 1:50~1:100.
Restrictions
For Research Use only
Format
Liquid
Concentration
100ug/100ul
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Freson, Devriendt, Matthijs, Van Hoof, De Vos, Thys, Minner, Hoylaerts, Vermylen, Van Geet: "Platelet characteristics in patients with X-linked macrothrombocytopenia because of a novel GATA1 mutation." in: Blood, Vol. 98, Issue 1, pp. 85-92, (2001) (PubMed).
Mehaffey, Newton, Gandhi, Crossley, Drachman: "X-linked thrombocytopenia caused by a novel mutation of GATA-1." in: Blood, Vol. 98, Issue 9, pp. 2681-8, (2001) (PubMed).
Nichols, Crispino, Poncz, White, Orkin, Maris, Weiss: "Familial dyserythropoietic anaemia and thrombocytopenia due to an inherited mutation in GATA1." in: Nature genetics, Vol. 24, Issue 3, pp. 266-70, (2000) (PubMed).
Zon, Tsai, Burgess, Matsudaira, Bruns, Orkin: "The major human erythroid DNA-binding protein (GF-1): primary sequence and localization of the gene to the X chromosome." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 2, pp. 668-72, (1990) (PubMed).
Trainor, Evans, Felsenfeld, Boguski: "Structure and evolution of a human erythroid transcription factor." in: Nature, Vol. 343, Issue 6253, pp. 92-6, (1990) (PubMed).
Target
GATA1
(GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))