GATA1 antibody (pSer142)

Catalog No. ABIN6254982

The Rabbit Polyclonal anti-GATA1 antibody is suitable to detect GATA1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IP, IF and ICC.

Quick Overview for GATA1 antibody (pSer142) (ABIN6254982)

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-GATA1 Antibody

Binding Specificity: pSer142

Purpose: Phospho-GATA1 (Ser142) Antibody

Specificity: Phospho-GATA1 (Ser142) Antibody detects endogenous levels of GATA1 only when phosphorylated at Serine 142.

Predicted Reactivity: Pig,Sheep,Rabbit

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human GATA1 around the phosphorylation site of Ser142.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for GATA1

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Alternative Name: GATA1

Background:

Description: Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781).

Gene: GATA1

Molecular Weight: 43kDa

Gene ID: 2623

UniProt: P15976

Pathways: Cellular Response to Molecule of Bacterial Origin