HSP27 antibody

Catalog No. ABIN966312

Cited in 5+ publications. The Rabbit Polyclonal anti-HSP27 antibody (ABIN966312) specifically detects HSP27 in IHC. The antibody is reactive with Human samples.

Quick Overview for HSP27 antibody (ABIN966312)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-HSP27 Antibody

Specificity: HSP27 (Ab-15) antibody detects endogenous levels of total HSP27 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human HSP27 around the phosphorylation site of serine 15 (G-P-SP-W-D).

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-HSP27 antibody (ABIN966312)

Scrimin, Axia, Tremolada, Pillon, Capello, Zanesco: "Conversational strategies with parents of newly diagnosed leukaemic children: an analysis of 4880 conversational turns." in: Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer, Vol. 13, Issue 5, pp. 287-94, (2005) (PubMed).

Tremolada, Magni, Valsecchi, Sarto, Mocarelli, Perego, Cordani, Favini, Galli Kienle, Sanchez, Hochstrasser, Corthals: "Characterization of heat shock protein 27 phosphorylation sites in renal cell carcinoma." in: Proteomics, Vol. 5, Issue 3, pp. 788-95, (2005) (PubMed).

McGregor, Kempster, Wait, Gosling, Dunn, Powell: "F-actin capping (CapZ) and other contractile saphenous vein smooth muscle proteins are altered by hemodynamic stress: a proteonomic approach." in: Molecular & cellular proteomics : MCP, Vol. 3, Issue 2, pp. 115-24, (2004) (PubMed).

Benn, Perrelet, Kato, Scholz, Decosterd, Mannion, Bakowska, Woolf: "Hsp27 upregulation and phosphorylation is required for injured sensory and motor neuron survival." in: Neuron, Vol. 36, Issue 1, pp. 45-56, (2002) (PubMed).

MacDonald, Mackey, Pearson, Haystead: "A strategy for the rapid identification of phosphorylation sites in the phosphoproteome." in: Molecular & cellular proteomics : MCP, Vol. 1, Issue 4, pp. 314-22, (2002) (PubMed).

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSP27

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling