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Heat Shock Protein 2 (HSPB2) antibody

Antigen

Heat Shock Protein 2 (HSPB2)
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
3 references available
Catalog no. ABIN966313
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name HSP27
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine 78 (A-L-SP-R-Q).
Specificity HSP27 (Ab-78) antibody detects endogenous levels of total HSP27 protein.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Jordan, Brownstone, Noga: "Control of functional systems in the brainstem and spinal cord." in: Current opinion in neurobiology, Vol. 2, Issue 6, pp. 794-801, 1993 (PubMed).

Landry, Lambert, Zhou et al.: "Human HSP27 is phosphorylated at serines 78 and 82 by heat shock and mitogen-activated kinases that recognize the same amino acid motif as S6 kinase II." in: The Journal of biological chemistry, Vol. 267, Issue 2, pp. 794-803, 1992 (PubMed).

Somara, Bitar: "Tropomyosin interacts with phosphorylated HSP27 in agonist-induced contraction of smooth muscle." in: American journal of physiology. Cell physiology, Vol. 286, Issue 6, pp. C1290-301, 2004 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Heat Shock Protein 2 (HSPB2)", type "Antibodies"
Hosts Rabbit (76), Mouse (18), Chicken (1)
Reactivities Human (96), Rat (Rattus) (33), Mouse (Murine) (25), Dog (Canine) (6), Horse (Equine) (6), Cow (Bovine) (5), Chimpanzee (1), Macaque (1)
Applications Western Blotting (WB) (63), Immunohistochemistry (IHC) (54), Immunofluorescence (IF) (31), ELISA (16), Flow Cytometry (FACS) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunoprecipitation (IP) (8), Immunocytochemistry (ICC) (5), Electrophoretic Mobility-Shift Assay (EMSA) (2)
Conjugates Biotin (6), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), FITC (3), DyLight 488 (1), PE (1)
Epitopes pSer78 (15), pSer15 (12), pSer82 (12), pSer254 (4), Ser15 (2), Ser78 (2), Ser82 (2), AA 50-100 (1), Center (1), pSer565 (1), pSer78, pSer82 (1), pTyr143 (1), pTyr452 (1)