conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody
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| Synonyms |
IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Ikka, Chuk1, Fbx24, Fbxo24, AI256658, MGC25325, CHUK, chuk, MGC83432, DKFZp459O053 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 488 (5), Alexa Fluor 350 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Biotin (3), Alexa Fluor (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
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Application
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Western Blotting (WB) (101), ELISA (59), Immunofluorescence (IF) (46), Immunohistochemistry (IHC) (21), Immunoprecipitation (IP) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (19), Immunocytochemistry (ICC) (11), Flow Cytometry (FACS) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN966383 |
| Quantity |
100ug (100ug/100ul.) |
| Price |
385.00 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 5 to 7 Business Days |
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Alternative name
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IKK alpha
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Immunogen
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The antiserum was produced against synthesized non-phosphopeptide derived from human IKK around the phosphorylation site of Threonine 23 (L-G-TP-G-G).
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Specificity
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IKK (Ab-23) Antibody detects endogenous levels of total IKK protein.
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Application Notes
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WB: 1:500~1:1000 IHC: 1:50~1:100.
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Concentration
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100ug/100ul.
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Buffer
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Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
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Preservative
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0.02% Sodium Azide
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Storage
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Store at -20°C/1 year.
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Restrictions
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For Research Use only
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Product
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Ozes, Mayo, Gustin et al.: "NF-kappaB activation by tumour necrosis factor requires the Akt serine-threonine kinase." in: Nature, Vol. 401, Issue 6748, pp. 82-5, 1999 (PubMed).
Yuan, Feldman, Sun et al.: "Inhibition of JNK by cellular stress- and tumor necrosis factor alpha-induced AKT2 through activation of the NF kappa B pathway in human epithelial Cells." in: The Journal of biological chemistry, Vol. 277, Issue 33, pp. 29973-82, 2002 (PubMed).
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Alternatives for antigen "conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK)", type "Antibodies"
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Hosts
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Rabbit (110), Mouse (28)
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Reactivities
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Human (135), Mouse (Murine) (46), Rat (Rattus) (46), Dog (Canine) (33), Horse (Equine) (28), Chicken (5), Cow (Bovine) (5), Pig (Porcine) (5)
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Applications
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Western Blotting (WB) (101), ELISA (59), Immunofluorescence (IF) (46), Immunohistochemistry (IHC) (21), Immunoprecipitation (IP) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (19), Immunocytochemistry (ICC) (11), Flow Cytometry (FACS) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Fluor 488 (5), Alexa Fluor 350 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Biotin (3), Alexa Fluor (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
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Epitopes
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pThr23 (18), AA 716-734 (8), AA 658-674 (6), C-Term (6), pTyr463 (5), AA 699-715 (4), pSer180,pSer176 (4), AA 557-745 (3), Thr23 (3), AA 500-590 (2), N-Term (2), Ser180, Ser181 (1), pSer176 (1), pSer176, pSer177 (1), pSer176, pSer180 (1), pSer31 (1), pTyr188 (1)
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