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conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody

Antigen

conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK)

Synonyms IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Ikka, Chuk1, Fbx24, Fbxo24, AI256658, MGC25325, CHUK, chuk, MGC83432, DKFZp459O053
Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)

Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
2 references available
Catalog no. ABIN966383
Quantity 100ug  (100ug/100ul.)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information

Alternative name IKK alpha
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human IKK around the phosphorylation site of Threonine 23 (L-G-TP-G-G).
Specificity IKK (Ab-23) Antibody detects endogenous levels of total IKK protein.

Application Details

Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications

Product Ozes, Mayo, Gustin et al.: "NF-kappaB activation by tumour necrosis factor requires the Akt serine-threonine kinase." in: Nature, Vol. 401, Issue 6748, pp. 82-5, 1999 (PubMed).

Yuan, Feldman, Sun et al.: "Inhibition of JNK by cellular stress- and tumor necrosis factor alpha-induced AKT2 through activation of the NF kappa B pathway in human epithelial Cells." in: The Journal of biological chemistry, Vol. 277, Issue 33, pp. 29973-82, 2002 (PubMed).

Alternatives

Alternatives for antigen "conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK)", type "Antibodies"
Hosts Rabbit (110), Mouse (28)
Reactivities Human (135), Mouse (Murine) (46), Rat (Rattus) (46), Dog (Canine) (33), Horse (Equine) (28), Chicken (5), Cow (Bovine) (5), Pig (Porcine) (5)
Applications Western Blotting (WB) (101), ELISA (59), Immunofluorescence (IF) (46), Immunohistochemistry (IHC) (21), Immunoprecipitation (IP) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (19), Immunocytochemistry (ICC) (11), Flow Cytometry (FACS) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 488 (5), Alexa Fluor 350 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Biotin (3), Alexa Fluor (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pThr23 (18), AA 716-734 (8), AA 658-674 (6), C-Term (6), pTyr463 (5), AA 699-715 (4), pSer180,pSer176 (4), AA 557-745 (3), Thr23 (3), AA 500-590 (2), N-Term (2), Ser180, Ser181 (1), pSer176 (1), pSer176, pSer177 (1), pSer176, pSer180 (1), pSer31 (1), pTyr188 (1)