Metadherin (MTDH) antibody

Antigen: Metadherin (MTDH)

Synonyms: 3D3, Lyric, AV353288, 3D3/Lyric, D8Bwg1112e, 2610103J23Rik, AEG1, AEG-1, LYRIC, LYRIC/3D3, MGC75963, MGC137491

Clonality: Monoclonal (2F11C3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Catalog no.: ABIN966573

Additional Information

Alternative name: Metadherin

Gene ID: 92140

Immunogen: Purified recombinant fragment of human Metadherin expressed in E. Coli.

Isotype: IgG1

Clone: 2F11C3

Description: Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain, blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.
Synonyms: 3D3, AEG1, LYRIC, MTDH

Molecular Weight: 64kDa

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Antibodies are purified by protein G affinity chromatography.

Buffer: PBS containing 50% glycerol

Preservative: 0.03% sodium azide.

Storage: Store at 4°C, for long term storage, store at -20°C.

Restrictions: For Research Use only

Publications

Product: Brown, Ruoslahti: "Metadherin, a cell surface protein in breast tumors that mediates lung metastasis." in: Cancer cell, Vol. 5, Issue 4, pp. 365-74, 2004 (PubMed).

Britt, Yang, Yang et al.: "Identification of a novel protein, LYRIC, localized to tight junctions of polarized epithelial cells." in: Experimental cell research, Vol. 300, Issue 1, pp. 134-48, 2004 (PubMed).

Méndez, Audicana, Cancer et al.: "Assessment of drinking water quality using indicator bacteria and bacteriophages." in: Journal of water and health, Vol. 2, Issue 3, pp. 201-14, 2004 (PubMed).

Hu, Chong, Yang et al.: "MTDH activation by 8q22 genomic gain promotes chemoresistance and metastasis of poor-prognosis breast cancer." in: Cancer cell, Vol. 15, Issue 1, pp. 9-20, 2008 (PubMed).