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Mitogen-Activated Protein Kinase 3 (MAPK3) antibody

Antigen

Mitogen-Activated Protein Kinase 3 (MAPK3)
Synonyms ERK1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, MGC20180, zERK1, fi06b09, wu:fi06b09, Tb08.10J17.940
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
4 references available
Catalog no. ABIN966753
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Alternative name p44/42 MAP Kinase
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human p44/42 MAP Kinase around the phosphorylation site of tyrosine 204 (T-E-YP-V-A).
Specificity P44/42 MAP Kinase (Ab-204) antibody detects endogenous levels of total p44/42 MAP Kinase protein.

Application Details
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Application Notes WB: 1:500~1:1000.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Hannken, Schroeder, Zahner et al.: "Reactive oxygen species stimulate p44/42 mitogen-activated protein kinase and induce p27(Kip1): role in angiotensin II-mediated hypertrophy of proximal tubular cells." in: Journal of the American Society of Nephrology : JASN, Vol. 11, Issue 8, pp. 1387-97, 2000 (PubMed).

Ihn, Tamaki: "Oncostatin M stimulates the growth of dermal fibroblasts via a mitogen-activated protein kinase-dependent pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 4, pp. 2149-55, 2000 (PubMed).

Perez, Nolan: "Simultaneous measurement of multiple active kinase states using polychromatic flow cytometry." in: Nature biotechnology, Vol. 20, Issue 2, pp. 155-62, 2002 (PubMed).

Yu, Mizumoto, Tokinaga et al.: "The inhibitory effects of sevoflurane on angiotensin II- induced, p44/42 mitogen-activated protein kinase-mediated contraction of rat aortic smooth muscle." in: Anesthesia and analgesia, Vol. 101, Issue 2, pp. 315-21, table of contents, 2005 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Mitogen-Activated Protein Kinase 3 (MAPK3)", type "Antibodies"
Hosts Rabbit (217), Mouse (30), Goat (7), Chicken (1), Sheep (1)
Reactivities Human (241), Rat (Rattus) (158), Mouse (Murine) (142), Cow (Bovine) (61), Dog (Canine) (59), Chicken (58), Pig (Porcine) (55), Guinea Pig (54), Horse (Equine) (54), Xenopus laevis (5), Fruit Fly (Drosophila melanogaster) (2), Monkey (2), Rabbit (2), Sheep (Ovine) (2), Cat (Feline) (1), Primate (1), Zebrafish (1), Zebrafish (Danio rerio) (1)
Applications Western Blotting (WB) (163), ELISA (96), Immunofluorescence (IF) (92), Immunohistochemistry (IHC) (45), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (44), Immunoprecipitation (IP) (34), Immunocytochemistry (ICC) (14), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Flow Cytometry (FACS) (6), Dot Blot (Dot) (4), Immunoelectron Microscopy (IEM) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Dot Blot (DB) (2), Functional Studies (Func) (1)
Conjugates Biotin (7), FITC (7), HRP (5), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), Gold (4), PE (4), PE,Cy3 (4), PE,Cy5 (4), PE,Cy5.5 (4), PE,Cy7 (4)
Epitopes C-Term (34), pTyr204 (30), pThr202,pTyr204 (18), pThr197,pThr202 (13), N-Term (12), pThr202 (10), Internal Region (8), pThr202, pTyr204 (8), Tyr204 (5), pThr202,pThr197 (5), pTyr204,pThr202 (5), C-Term,AA 365-379 (4), Thr202 (4), pThr202, pThr204 (4), pThr-Glu-pTyr (3), Asp192 (2), pThr185, pTyr187 (2), AA 174-183 (1), AA 324-345 (1), AA 34-0 (1), AA 350-400 (1), C-Term,AA 352-380 (1), Tyr202 (1), pT-E-pY Motif (1)