CD9 antibody

Catalog No. ABIN967348

Product Details anti-CD9 Antibody

Target: CD9

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD9 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Not Reported

Clone: RPM-7

Isotype: IgG3 kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD9 antibody (ABIN967348)

Claas, Wahl, Orlicky, Karaduman, Schnölzer, Kempf, Zöller: "The tetraspanin D6.1A and its molecular partners on rat carcinoma cells." in: The Biochemical journal, Vol. 389, Issue Pt 1, pp. 99-110, (2005) (PubMed).

Terada, Baracskay, Kinter, Melrose, Brophy, Boucheix, Bjartmar, Kidd, Trapp: "The tetraspanin protein, CD9, is expressed by progenitor cells committed to oligodendrogenesis and is linked to beta1 integrin, CD81, and Tspan-2." in: Glia, Vol. 40, Issue 3, pp. 350-9, (2002) (PubMed).

Park, Yashiro, Tai, Toyo-oka, Hamaoka, Yagita, Okumura, Neben, Fujiwara: "Differential involvement of a Fas-CPP32-like protease pathway in apoptosis of TCR/CD9-costimulated, naive T cells and TCR-restimulated, activated T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 12, pp. 5790-6, (1998) (PubMed).

Kaprielian, Cho, Hadjiargyrou, Patterson: "CD9, a major platelet cell surface glycoprotein, is a ROCA antigen and is expressed in the nervous system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 1 Pt 2, pp. 562-73, (1995) (PubMed).

Wright, Tomlinson: "The ins and outs of the transmembrane 4 superfamily." in: Immunology today, Vol. 15, Issue 12, pp. 588-94, (1995) (PubMed).

Jennings, Crossno, Fox, White, Green: "Platelet p24/CD9, a member of the tetraspanin family of proteins." in: Annals of the New York Academy of Sciences, Vol. 714, pp. 175-84, (1994) (PubMed).

Target Details for CD9

Target: CD9

Alternative Name: CD9 (CD9 Products)

Background: The RPM.7 antibody reacts with CD9, a 26-kDa member of the transmembrane 4 superfamily. Rat CD9 mRNA is detected in many adult tissues, including neural and hematopoietic tissue. Using RPM.7 mAb in flow cytometric analysis of rat hematopoietic tissues, CD9 is found on platelets, peripheral B- and T-lymphocyte subsets, and leukocyte subpopulations in the bone marrow. In humans, CD9 has been found to be associated with integrins and other cell-surface receptors, and it is suggested to play a role in signal transduction and possibly in regulating cellular adhesive properties. In mice, CD9 has been demonstrated to participate in T-cell costimulation and induction of apoptosis.

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization