Lysosomal-Associated Membrane Protein 1 (LAMP1) antibody

Antigen: Lysosomal-Associated Membrane Protein 1 (LAMP1)

Synonyms: LAMPA, CD107a, LGP120

Clonality: Monoclonal (1D4B)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Intracellular Flow Cytometry (ICFC), Immunoelectron Microscopy (IEM), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Immunoaffinity Chromatography (IAC)

Catalog no.: ABIN967382

Additional Information

Alternative name: CD107a

Immunogen: Plasma membrane fraction of mouse embryo NIH 3T3 cell line

Format: Liquid

Isotype: IgG2a, kappa

Clone: 1D4B

Description: The 1D4B antibody recognizes CD107a (Lysosome-Associated Membrane Protein 1, LAMP-1), one of the two major glycoproteins in lysosome membranes which are useful markers to distinguish lysosomes from other organelles. Mouse CD107a consists of a 40-kDa core protein which is heavily glycosylated to form heterogeneous mature glycoprotein of 110-140 kDa. It is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice. Several lines of evidence suggest that cell-surface mouse and human LAMP-1 glycoproteins, the expression of which may correlate with malignant transformation, participate in intercellular adhesion and adhesion to extracellular matrix.
Synonyms: LAMP-1

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Application Details

Application Notes: This antibody conjugate has been tested by intracellular immunofluorescent staining ( Less or equal than 1 µg/million cells, using the Cytofix/Cytoperm™ Kit) with flow cytometric analysis to assure specificity and reactivity.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4° C.

Restrictions: For Research Use only

Publications

Arterburn, Earles, August: "The disulfide structure of mouse lysosome-associated membrane protein 1." in: The Journal of biological chemistry, Vol. 265, Issue 13, pp. 7419-23, 1990 (PubMed).

Chen, Chen, DSouza et al.: "Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2." in: Biochemical Society symposium, Vol. 51, pp. 97-112, 1987 (PubMed).

Chen, Pan, DSouza et al.: "Lysosome-associated membrane proteins: characterization of LAMP-1 of macrophage P388 and mouse embryo 3T3 cultured cells." in: Archives of biochemistry and biophysics, Vol. 239, Issue 2, pp. 574-86, 1985 (PubMed).

Rohrer, Schweizer, Russell et al.: "The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane." in: The Journal of cell biology, Vol. 132, Issue 4, pp. 565-76, 1996 (PubMed).