MADCAM1 antibody

Catalog No. ABIN967383

This anti-MADCAM1 antibody is a Rat Monoclonal antibody detecting MADCAM1 in WB, FACS, IHC (fro), IP, IAC and BR. Suitable for Mouse. This Primary Antibody has been cited in 9+ publications.

Quick Overview for MADCAM1 antibody (ABIN967383)

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MADCAM1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunoaffinity Chromatography (IAC), Blocking Reagent (BR)

Clone: MECA-367

Product Details anti-MADCAM1 Antibody

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes.

Isotype: IgG2a kappa

Application Details

Application Notes: For IHC, we recommend the use of purified MECA-367 mAb in our special formulation for immunohistochemistry.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-MADCAM1 antibody (ABIN967383)

Yang, Sytwu, McDevitt, Michie: "Involvement of beta 7 integrin and mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in the development of diabetes in obese diabetic mice." in: Diabetes, Vol. 46, Issue 10, pp. 1542-7, (1997) (PubMed).

Hänninen, Salmi, Simell, Andrew, Jalkanen: "Recirculation and homing of lymphocyte subsets: dual homing specificity of beta 7-integrin(high)-lymphocytes in nonobese diabetic mice." in: Blood, Vol. 88, Issue 3, pp. 934-44, (1996) (PubMed).

Kraal, Schornagel, Streeter, Holzmann, Butcher: "Expression of the mucosal vascular addressin, MAdCAM-1, on sinus-lining cells in the spleen." in: The American journal of pathology, Vol. 147, Issue 3, pp. 763-71, (1995) (PubMed).

Berg, McEvoy, Berlin, Bargatze, Butcher: "L-selectin-mediated lymphocyte rolling on MAdCAM-1." in: Nature, Vol. 366, Issue 6456, pp. 695-8, (1994) (PubMed).

Hamann, Andrew, Jablonski-Westrich, Holzmann, Butcher: "Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3282-93, (1994) (PubMed).

Lee, Sarvetnick: "Induction of vascular addressins and adhesion molecules in the pancreas of IFN-gamma transgenic mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 9, pp. 4597-603, (1994) (PubMed).

Berlin, Berg, Briskin, Andrew, Kilshaw, Holzmann, Weissman, Hamann, Butcher: "Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1." in: Cell, Vol. 74, Issue 1, pp. 185-95, (1993) (PubMed).

Briskin, McEvoy, Butcher: "MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1." in: Nature, Vol. 363, Issue 6428, pp. 461-4, (1993) (PubMed).

Streeter, Berg, Rouse, Bargatze, Butcher: "A tissue-specific endothelial cell molecule involved in lymphocyte homing." in: Nature, Vol. 331, Issue 6151, pp. 41-6, (1988) (PubMed).

Target Details for MADCAM1

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Alternative Name: MAdCAM-1

Background: The MECA-367 antibody reacts with mucosal vascular addressin MAdCAM-1. In the fetus and neonate, MAdCAM-1 is the predominant vascular addressin on the high endothelial venules (HEV) of peripheral lymph nodes. In adult mice, MAdCAM-1 is preferentially expressed in mucosal lymphoid tissues and lamina propria, it is also expressed on sinus-lining cells in the spleen. MAdCAM-1 expression is upregulated on the HEV of peripheral lymph nodes in adult NOD mice4 and is involved in the development of diabetes and insulitis. Furthermore, there is evidence that IFN-gamma can induce MAdCAM-1 expression in non-mucosal sites in adult mice. MAdCAM-1 is a predominant ligand for integrin alpha4beta7, a lymphocyte mucosal homing receptor, and a facultative ligand for CD62L (L-selectin). MECA-367 mAb binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro and lymphocyte homing to Peyer’s patch HEV in vivo. Source of the immunogen was endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes.