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Deleted in Colorectal Carcinoma (DCC) antibody

Details for Product No. ABIN967443
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Antigen
Synonyms CRC18, CRCR1, IGDCC1, MRMV1, Igdcc1, C030036D22Rik, Abcc1b, DCC, Dyscalc1, Mrp6, dyscalc, zdcc, xdcc, XDCCa, dcca-A
Reactivity
Human
(40), (35), (23), (3), (1), (1)
Host
Mouse
(51), (3), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
(30), (27), (10), (10), (9), (8), (3), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN967443
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Immunogen Recombinant Human DCC
Clone G97-449
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name DCC
Background One of the most common regions of allelic loss in colorectal tumors is chromosome 18, which is lost in more than 70% of carcinomas, and in almost 50% of late adenomas. This region of loss has been mapped to chromosome 18q and a gene called Deleted in Colorectal Cancer (DCC). DCC encodes an ~185 kDa glycoprotein with significant homology to the neural cell adhesion molecule and other related cell surface glycoproteins. The predicted amino acid sequence of DCC cDNA consists of a 1448 amino acid (aa) long transmembrane phosphoprotein. The extracellular domain consists of 1098 amino acids and has 42% sequence homology to cell adhesion proteins of the neural cell adhesion molecule (N-CAM) family. DCC mRNA is found to be expressed in normal colonic mucosa, but its expression is reduced or absent in the majority of colorectal carcinomas. The loss of heterozygosity and subsequent alteration of DCC expression has also been observed in tumors of non-colorectal origin. Clone G97-449 recognizes human DCC. A truncated recombinant protein containing the intracellular domain of the human DCC was used as immunogen.
Molecular Weight 175-190 kDa
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Apoptosis/Necrosis
Application Notes Applications include western blot analysis (0.5 - 2.0 µg/ml). IMR-32 cells are suggested as a positive control. Other applications not routinely tested include flow cytometric analysis (titrate between 0.06-1.0 µg/one million cells). The antibody has also been published for immunohistochemistry of formalin-fixed, paraffin-embedded tissue sections. By western blot, DCC-specific antibodies typically identify protein species with molecular weights of approximately 175-190 kDa. Doublets in this range have been reported in brain. Several smaller immunoreactive species, representing degradation products, cross-reactive species, or DCC forms arising from alternative splicing of DCC mRNA or in vivo processing of the DCC protein may also be identified.
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Deleted in Colorectal Carcinoma (DCC) antibody Western blot analysis of human DCC protein in 293 human embryonic kidney cells stably transfected with an expression vector containing full length DCC cDNA. Lane 1, clone G97-449 (ABIN967443), which recognizes an epitope in the intracellular domain of DCC. Lane 2, clone G92-13 (ABIN967442), which recognizes an epitope in the extracellular domain of DCC. Lane 3, a mouse IgG1 isotype control.
anti-Deleted in Colorectal Carcinoma (DCC) antibody (2) anti-Deleted in Colorectal Carcinoma (DCC) antibody (Image 2)
General Fearon, Cho, Nigro et al.: "Identification of a chromosome 18q gene that is altered in colorectal cancers." in: Science (New York, N.Y.), Vol. 247, Issue 4938, pp. 49-56, 1990 (PubMed).

Vogelstein, Fearon, Hamilton et al.: "Genetic alterations during colorectal-tumor development." in: The New England journal of medicine, Vol. 319, Issue 9, pp. 525-32, 1988 (PubMed).

Fearon, Hamilton, Vogelstein: "Clonal analysis of human colorectal tumors." in: Science (New York, N.Y.), Vol. 238, Issue 4824, pp. 193-7, 1987 (PubMed).

Reale, Hu, Zafar et al.: "Expression and alternative splicing of the deleted in colorectal cancer (DCC) gene in normal and malignant tissues." in: Cancer research, Vol. 54, Issue 16, pp. 4493-501, 1994 (PubMed).

Shibata, Reale, Lavin et al.: "The DCC protein and prognosis in colorectal cancer." in: The New England journal of medicine, Vol. 335, Issue 23, pp. 1727-32, 1996 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN967443
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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