FLI1 antibody

Catalog No. ABIN967450

This Mouse Monoclonal antibody specifically detects FLI1 in WB and IP. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for FLI1 antibody (ABIN967450)

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FLI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: G146-222

Product Details anti-FLI1 Antibody

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Fli-1 ets Domain Fusion Protein

Isotype: IgG2b

Application Details

Application Notes: G146-222 has been shown to recognize in vitro translated, recombinant bacterially expressed and endogenous B cell Fli-1. In gel shift assays, this antibody disrupts complexes of Fli-1 and DNA probe, however, this application is not tested.

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-FLI1 antibody (ABIN967450)

Bailly, Bosselut, Zucman, Cormier, Delattre, Roussel, Thomas, Ghysdael: "DNA-binding and transcriptional activation properties of the EWS-FLI-1 fusion protein resulting from the t(11;22) translocation in Ewing sarcoma." in: Molecular and cellular biology, Vol. 14, Issue 5, pp. 3230-41, (1994) (PubMed).

May, Lessnick, Braun, Klemsz, Lewis, Lunsford, Hromas, Denny: "The Ewing's sarcoma EWS/FLI-1 fusion gene encodes a more potent transcriptional activator and is a more powerful transforming gene than FLI-1." in: Molecular and cellular biology, Vol. 13, Issue 12, pp. 7393-8, (1994) (PubMed).

Target Details for FLI1

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Alternative Name: Fli-1

Background: Fli-1 is a 50 kDa ets-related transcription factor. Like other ets-related proteins it binds to consensus sites in DNA through an 85 amino acid ets domain in the carboxyl region of the protein. Fli-1 is associated with Ewing sarcoma (EWS) through a t(11,22)(q24,q12) chromosomal translocation. This translocation brings the 5' region of the EWS gene into conjunction with the 3' region of the Fli-1 gene encoding the ets-DNA binding domain. Such a translocation is found in 85% of Ewing sarcomas. The resulting fusion protein has the DNA binding activity of Fli- 1 and, with the EWS domain, it is also a more potent transcriptional activator than Fli-1 itself. The strong transforming activity of the EWS-Fli-1 fusion protein may in part be due to its ability to transactivate the promoter for c-myc. G146-222 recognizes human Fli-1. The antibody was raised against a bacterially expressed Fli-1 ets domain fusion protein.

Molecular Weight: 50 kDa