Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (pTyr774) antibody

Details for Product No. ABIN967577
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Antigen
Synonyms 4732447J05Rik, Cbl-2, c-Cbl, cbl, C-CBL, CBL2, FRA11B, NSLL, RNF55, c-cbl-like, RGD1561386
Epitope
pTyr774
(32), (31), (22), (21), (19), (5), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(232), (142), (139), (63), (51), (51), (24), (1), (1), (1)
Host
Mouse
(206), (20), (10), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application
Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
(163), (74), (72), (60), (57), (41), (18), (18), (15), (9), (4), (3), (2), (2), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN967577
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Immunogen Phosphorylated Human c-Cbl Peptide
Clone OPG-01
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name c-Cbl
Background Cbl (Casitas B-lineage lymphoma) was identified in the genome of a transforming retrovirus from a mouse pre-B lymphoma. The cellular gene product c-Cbl is one of numerous Cbl-related proteins found in vertebrate and invertebrate organisms. It is an 120-kDa adapter protein that contains multiple functional domains, including a RING finger motif, a tyrosine kinase-binding (TKB) domain, and a proline-rich region. The TKB domain directly interacts with specific auto-phosphorylation sites in activated protein-tyrosine kinases (PTK). Through the RING finger motif, c-Cbl recruits and activates an E2 ubiquitin-conjugating enzyme, thus targeting the activated PTK for protein degradation. The proline-rich region contains SH3 domain-binding and 14-3-3 protein-binding motifs. c-Cbl is also phosphorylated at tyrosines 700, 731, and 774 (Y774) by Syk- and Src-family kinases after the stimulation of some integrins and a wide variety of receptors for antigens, immunoglobulins, growth factors, cytokines, and hormones. In turn, the phosphorylated Y774 site interacts with the SH2 domain of the CRK adapter protein. The c-Cbl adapter protein is expressed in the cytoplasm in all tissues, with especially high levels of expression in hematopoietic cells. Through its many functional sites, c-Cbl plays key roles in the positive and negative regulation of vital cell functions, including T Cell Receptor-mediated cellular immune responses. The 29/c-Cbl monoclonal antibody recognizes the Y774-phosphorylated form of human c-Cbl.
Comment

Related Products: ABIN967389, ABIN968537, ABIN968656

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (pTyr774) antibody Western blot analysis of c-Cbl (pY774) in human T leukemia. Lysates from control (ABIN968537, left panel) and Pervanadate-treated (ABIN968656, right panel) Jurkat cells were probed with purified mouse anti-c-Cbl (pY774) monoclonal antibody at concentrations of 1.0, 0.5, and 0.25 myg/ml (Lanes 1, 2, and 3, respectively). c-Cbl (pY774) is identified as a band of 120 kDa in the treated cells.
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (pTyr774) antibody (2) c-Cbl (pY774) staining on tonsil. Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed. Following antigen retrieval with Retrievagen A buffer, the sections were either left untreated (left panel) or treated with a phosphatase to eliminate all phosphorylation (right panel). The tissue sections were stained with purified mouse anti-c-Cbl (pY774) with Hematoxylin counterstaining. Original magnification: 20X.
Product cited in: Thien, Langdon: "Cbl: many adaptations to regulate protein tyrosine kinases." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 4, pp. 294-307, 2001 (PubMed).

Tsygankov, Teckchandani, Feshchenko et al.: "Beyond the RING: CBL proteins as multivalent adapters." in: Oncogene, Vol. 20, Issue 44, pp. 6382-402, 2001 (PubMed).

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