B-Cell Linker antibody (pTyr84)

Catalog No. ABIN967596

This anti-B-Cell Linker antibody is a Mouse Monoclonal antibody detecting B-Cell Linker in WB. Suitable for Human and Mouse. This Primary Antibody has been cited in 5+ publications.

Quick Overview for B-Cell Linker antibody (pTyr84) (ABIN967596)

Target: B-Cell Linker (BLNK)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This B-Cell Linker antibody is un-conjugated

Application: Western Blotting (WB)

Clone: J117-1278

Product Details anti-B-Cell Linker Antibody

Binding Specificity: pTyr84

Brand: BD Pharmingen™

Cross-Reactivity: Mouse (Murine)

Characteristics: 1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human BLNK Peptide

Isotype: IgG2b kappa

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-B-Cell Linker antibody (ABIN967596)

Pighi, Gu, Dalai, Barbi, Parolini, Bertolaso, Pedron, Parisi, Ren, Cecconi, Chilosi, Menestrina, Zamò: "Phospho-proteomic analysis of mantle cell lymphoma cells suggests a pro-survival role of B-cell receptor signaling." in: Cellular oncology (Dordrecht), Vol. 34, Issue 2, pp. 141-53, (2011) (PubMed).

Espeli, Mancini, Breton, Poirier, Schiff: "Impaired B-cell development at the pre-BII-cell stage in galectin-1-deficient mice due to inefficient pre-BII/stromal cell interactions." in: Blood, Vol. 113, Issue 23, pp. 5878-86, (2009) (PubMed).

Wu, Koretzky: "The SLP-76 family of adapter proteins." in: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, (2004) (PubMed).

Janssen, Zhang: "Adaptor proteins in lymphocyte activation." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, (2003) (PubMed).

Chiu, Dalton, Ishiai, Kurosaki, Chan: "BLNK: molecular scaffolding through 'cis'-mediated organization of signaling proteins." in: The EMBO journal, Vol. 21, Issue 23, pp. 6461-72, (2002) (PubMed).

Target Details for B-Cell Linker

Target: B-Cell Linker (BLNK)

Alternative Name: BLNK

Background: B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The J117-1278 monoclonal antibody recognizes the phosphorylated Y84 of BLNK.

Molecular Weight: 68 kDa

Pathways: BCR Signaling