ROR gamma T/RORC2/NR1F3 antibody

Catalog No. ABIN967684

This Mouse Monoclonal antibody specifically detects in WB. It exhibits reactivity toward Human and Mouse and has been mentioned in 6+ publications.

Quick Overview for ROR gamma T/RORC2/NR1F3 antibody (ABIN967684)

Target: ROR gamma T/RORC2/NR1F3

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Clone: O28-835

Product Details

Brand: BD Pharmingen™

Cross-Reactivity: Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human RORgammat Recombinant Protein

Isotype: IgG2b kappa

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Hamada, Garcia-Hernandez, Reome, Misra, Strutt, McKinstry, Cooper, Swain, Dutton: "Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 6, pp. 3469-81, (2009) (PubMed).

Ivanov, McKenzie, Zhou, Tadokoro, Lepelley, Lafaille, Cua, Littman: "The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells." in: Cell, Vol. 126, Issue 6, pp. 1121-33, (2006) (PubMed).

Eberl, Littman: "The role of the nuclear hormone receptor RORgammat in the development of lymph nodes and Peyer's patches." in: Immunological reviews, Vol. 195, Issue 9, pp. 81-90, (2003) (PubMed).

Eberl, Marmon, Sunshine, Rennert, Choi, Littman: "An essential function for the nuclear receptor RORgamma(t) in the generation of fetal lymphoid tissue inducer cells." in: Nature immunology, Vol. 5, Issue 1, pp. 64-73, (2003) (PubMed).

He, Deftos, Ojala, Bevan: "RORgamma t, a novel isoform of an orphan receptor, negatively regulates Fas ligand expression and IL-2 production in T cells." in: Immunity, Vol. 9, Issue 6, pp. 797-806, (1999) (PubMed).

Medvedev, Yan, Hirose, Giguère, Jetten: "Cloning of a cDNA encoding the murine orphan receptor RZR/ROR gamma and characterization of its response element." in: Gene, Vol. 181, Issue 1-2, pp. 199-206, (1997) (PubMed).

Target Details

Target: ROR gamma T/RORC2/NR1F3

Alternative Name: ROR GAM T

Background: The O28-835 monoclonal antibody recognizes ROR-gamma (RORgamma) and its isoform, RORgammat. RORgamma (~58 kDa calculated molecular weight) is a DNA-binding transcription factor that belongs to the ROR/RZR orphan nuclear receptor family. It is expressed in different tissues including skeletal muscle, kidney, liver and adipose tissues. RORgammat (~56 kDa calculated molecular weight) is a RORgamma isoform that is expressed exclusively by lymphoid cells. It is expressed by various lymphoid cell types including CD4+CD8+ thymocytes, peripheral CD4+ Th17 and CD8+ Tc17 cells and lymphoid tissue inducer (Lti) cells. RORgammat plays essential roles in thymopoiesis, T cell homeostasis, differentiation of effector T lymphocytes and the development of secondary lymphoid tissues including lymph nodes and Peyer’s patches.
Synonyms: Rorc, Rorg, RORgamma, Rorgamma, RORgt, RORgammat, RORgammat, TOR, Thor, Nr1f3, ROR

Molecular Weight: 56-58 kDa