Fibronectin 1 (FN1) antibody

Antigen: Fibronectin 1 (FN1)

Synonyms: FN, CIG, FNZ, MSF, ED-B, FINC, GFND, LETS, GFND2, DKFZp686H0342, DKFZp686I1370, DKFZp686F10164, DKFZp686O13149, Fn, Fn-1, MGC117493, E330027I09, fn-1, FIBNEC, FN1, cig, msf, finc, lets, MGC97508, fibronectin, fn2, fb80d10, wu:fb80d10

Clonality: Monoclonal (10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Catalog no.: ABIN967729

Additional Information

Alternative name: Fibronectin

Immunogen: Human Fibronectin

Cross-Reactivity: Cow (Bovine), Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 10

Description: The 240 kDa dimeric fibronectin protein exists in two forms: a soluble protomer in body fluids and an insoluble multimer in the extracellular matrix. The latter is the primary functional form and creates a substrate for cell migration, a role which makes fibronectin vital to embryogenesis and wound response. Fibronectin mediates cytoskeletal organization, cell attachment, and cellular signaling through interactions with cellular receptors. Although various isoforms of fibronectin are derived by alternative splicing, they share a common N-terminus which is a critical region for cell surface binding in an initial step of multimer assembly. Further polymerization steps are regulated by fibronectin/integrin interactions and result in generation of the complex fibrils that constitute the fibronectin matrix.
Synonyms: FN, LETS

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. For fluorochrome spectra and suitable instrument settings, please refer to us.

Molecular Weight: 240 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Synonyms: FN, CIG, FNZ, MSF, ED-B, FINC, GFND, LETS, GFND2, DKFZp686H0342, DKFZp686I1370, DKFZp686F10164, DKFZp686O13149, Fn, Fn-1, MGC117493, E330027I09, fn-1, FIBNEC, FN1, cig, msf, finc, lets, MGC97508, fibronectin, fn2, fb80d10, wu:fb80d10

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Research Area: Lineage Markers, Extracellular Matrix

Restrictions: For Research Use only

Images

Western blot analysis of Fibronectin. A-431 (ATCC CRL-1555) cell lysate was blotted at the follwing dilutions: Lane 1: 1:5000, Lane 2: 1:10,000, Lane 3: 1:20,000.

Immunofluorescent analysis of Fibronectin in mesenchymal stem cells (MSC). MSC (Lonza), passage 6, grown in BD Mosaic™ hMSC Serum Free Cell Culture Environment, were fixed in BD Cytofix™ Fixation Buffer, permeabilized with 0.1% Triton™ X-100 and stained with mouse anti-Fibronectin monoclonal antibody (ABIN967728, pseudo-colored green) at 2.5 µg/ml. Counter-staining of cell nuclei was with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™Software.

Publications

Product: Chen, Mosher: "Formation of sodium dodecyl sulfate-stable fibronectin multimers. Failure to detect products of thiol-disulfide exchange in cyanogen bromide or limited acid digests of stabilized matrix fibronectin." in: The Journal of biological chemistry, Vol. 271, Issue 15, pp. 9084-9, 1996 (PubMed).

Sechler, Takada, Schwarzbauer: "Altered rate of fibronectin matrix assembly by deletion of the first type III repeats." in: The Journal of cell biology, Vol. 134, Issue 2, pp. 573-83, 1996 (PubMed).

Zuk, Bonventre, Brown et al.: "Polarity, integrin, and extracellular matrix dynamics in the postischemic rat kidney." in: The American journal of physiology, Vol. 275, Issue 3 Pt 1, pp. C711-31, 1998 (PubMed).

Rhee, Sen, Lu et al.: "Wnt and frizzled receptors as potential targets for immunotherapy in head and neck squamous cell carcinomas." in: Oncogene, Vol. 21, Issue 43, pp. 6598-605, 2002 (PubMed).

Danen, Sonneveld, Brakebusch et al.: "The fibronectin-binding integrins alpha5beta1 and alphavbeta3 differentially modulate RhoA-GTP loading, organization of cell matrix adhesions, and fibronectin fibrillogenesis." in: The Journal of cell biology, Vol. 159, Issue 6, pp. 1071-86, 2002 (PubMed).