Protein Kinase C, alpha (PKCa) (AA 270-427) antibody

Antigen: Protein Kinase C, alpha (PKCa)

Synonyms: AAG6, PKCA, PRKACA, MGC129900, MGC129901, PKC-alpha, Pkca, AI875142, PRKCA, PKRCA, prkca, MGC82897, im:7139045, si:dkey-8l19.1, pkcalpha, MGC146825, pkc-alpha, LOC100219195

Binding Site: AA 270-427

Clonality: Monoclonal (3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Catalog no.: ABIN967750

Additional Information

Alternative name: PKC alpha

Immunogen: Human PKCalpha

Cross-Reactivity: Rat (Rattus), Mouse (Murine), Chicken, Dog (Canine), Frog

Format: Liquid

Isotype: IgG2b

Clone: 3

Description: The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members ( delta, epsilon, eta, and theta isoforms) and the atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) are Ca2+ independent and lack the C2 domain. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. Overexpression of PKCalpha has been reported to lead to an enhanced growth rate and induces the phosphorylation of two cellular proteins of 52 kDa and 90 kDa. This antibody is routinely tested by western blot analysis.
This antibody has been reported to also crossreact with PKCbeta.
Synonyms: Protein Kinase Calpha

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 82 kDa

Comments:

Related Products: ABIN968545, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Cardiovascular, Hypertrophy, Signaling, Protein Modifications, Kinases/Phosphatases

Restrictions: For Research Use only

Images

Western blot analysis of PKCalpha on a rat cerebrum lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-PKCalpha antibody.

Immunofluorescence staining of PC12 cells (Rat neuroblastoma, ATCC CRL-1721).

Publications

Product: Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." in: Nature, Vol. 334, Issue 6184, pp. 661-5, 1988 (PubMed).

Smart, Ying, Anderson: "Hormonal regulation of caveolae internalization." in: The Journal of cell biology, Vol. 131, Issue 4, pp. 929-38, 1996 (PubMed).

Zhang, Bontrager, Hemler: "Transmembrane-4 superfamily proteins associate with activated protein kinase C (PKC) and link PKC to specific beta(1) integrins." in: The Journal of biological chemistry, Vol. 276, Issue 27, pp. 25005-13, 2001 (PubMed).

Powner, Hodgkin, Wakelam: "Antigen-stimulated activation of phospholipase D1b by Rac1, ARF6, and PKCalpha in RBL-2H3 cells." in: Molecular biology of the cell, Vol. 13, Issue 4, pp. 1252-62, 2002 (PubMed).

Wagner, Fowler, Takemoto: "The interaction and phosphorylation of tropomodulin by protein kinase Calpha in N/N 1003A lens epithelial cells." in: Molecular vision, Vol. 8, pp. 394-406, 2002 (PubMed).

Fujino, Wu, Lin et al.: "cpg15 and cpg15-2 constitute a family of activity-regulated ligands expressed differentially in the nervous system to promote neurite growth and neuronal survival." in: The Journal of comparative neurology, Vol. 507, Issue 5, pp. 1831-45, 2008 (PubMed).