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Details for Product No. ABIN967750

Protein Kinase C, alpha (PKCa) (AA 270-427) antibody

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Antigen
Synonyms AAG6, PKC-alpha, PKCA, PRKACA, Pkca, AI875142, PKRCA, pkc-alpha, pkcalpha, im:7139045, si:dkey-8l19.1, LOC100219195
Epitope
»Alternatives AA 270-427
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Pubmed 6 references available
Catalog no. ABIN967750
Quantity 50 µg
Price
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Immunogen Human PKCalpha
Clone 3
Isotype IgG2b
Cross-Reactivity Rat (Rattus), Mouse (Murine), Chicken, Dog (Canine), Frog
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name PKC alpha
Background The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members ( delta, epsilon, eta, and theta isoforms) and the atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) are Ca2+ independent and lack the C2 domain. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. Overexpression of PKCalpha has been reported to lead to an enhanced growth rate and induces the phosphorylation of two cellular proteins of 52 kDa and 90 kDa. This antibody is routinely tested by western blot analysis.
This antibody has been reported to also crossreact with PKCbeta.
Synonyms: Protein Kinase Calpha
Molecular Weight 82 kDa
Research Area Cardiovascular, Hypertrophy, Signaling, Protein Modifications, Kinases/Phosphatases
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Product cited in: Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." in: Nature, Vol. 334, Issue 6184, pp. 661-5, 1988 (PubMed).

Smart, Ying, Anderson: "Hormonal regulation of caveolae internalization." in: The Journal of cell biology, Vol. 131, Issue 4, pp. 929-38, 1996 (PubMed).

Zhang, Bontrager, Hemler: "Transmembrane-4 superfamily proteins associate with activated protein kinase C (PKC) and link PKC to specific beta(1) integrins." in: The Journal of biological chemistry, Vol. 276, Issue 27, pp. 25005-13, 2001 (PubMed).

Powner, Hodgkin, Wakelam: "Antigen-stimulated activation of phospholipase D1b by Rac1, ARF6, and PKCalpha in RBL-2H3 cells." in: Molecular biology of the cell, Vol. 13, Issue 4, pp. 1252-62, 2002 (PubMed).

Wagner, Fowler, Takemoto: "The interaction and phosphorylation of tropomodulin by protein kinase Calpha in N/N 1003A lens epithelial cells." in: Molecular vision, Vol. 8, pp. 394-406, 2002 (PubMed).

Fujino, Wu, Lin et al.: "cpg15 and cpg15-2 constitute a family of activity-regulated ligands expressed differentially in the nervous system to promote neurite growth and neuronal survival." in: The Journal of comparative neurology, Vol. 507, Issue 5, pp. 1831-45, 2008 (PubMed).

Alternatives for antigen "Protein Kinase C, alpha (PKCa)", type "Antibodies"
Hosts (153), (20), (1)
Reactivities (159), (104), (94), (27), (24), (24), (12), (3), (2), (1)
Applications (133), (81), (41), (39), (30), (30), (20), (18), (12), (10), (2), (2), (2), (1), (1), (1), (1), (1)
Conjugates (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1)
Epitopes (25), (14), (13), (12), (9), (8), (6), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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