CNTNAP1 antibody (AA 1-1513)

Catalog No. ABIN967781

The Mouse Monoclonal anti-CNTNAP1 antibody has been validated for WB, IHC, IP and IF. It is suitable to detect CNTNAP1 in samples from Human, Mouse, Rat, Dog, Chicken and Frog. There are 5+ publications available.

Quick Overview for CNTNAP1 antibody (AA 1-1513) (ABIN967781)

Target: CNTNAP1 (Contactin Associated Protein 1 (CNTNAP1))

Reactivity: Human, Mouse, Rat, Dog, Chicken, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CNTNAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 30-p190

Product Details anti-CNTNAP1 Antibody

Binding Specificity: AA 1-1513

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken, Frog

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human p190 aa. 1-1513

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-CNTNAP1 antibody (ABIN967781)

Maddox, Burridge: "RhoA is required for cortical retraction and rigidity during mitotic cell rounding." in: The Journal of cell biology, Vol. 160, Issue 2, pp. 255-65, (2003) (PubMed).

Wolf, Draghi, Liang, Dai, Uhrbom, Eklöf, Westermark, Holland, Resh: "p190RhoGAP can act to inhibit PDGF-induced gliomas in mice: a putative tumor suppressor encoded on human chromosome 19q13.3." in: Genes & development, Vol. 17, Issue 4, pp. 476-87, (2003) (PubMed).

Brandt, Gimona, Hillmann, Haller, Mischak: "Protein kinase C induces actin reorganization via a Src- and Rho-dependent pathway." in: The Journal of biological chemistry, Vol. 277, Issue 23, pp. 20903-10, (2002) (PubMed).

Nakahara, Mueller, Nomizu, Yamada, Yeh, Chen: "Activation of beta1 integrin signaling stimulates tyrosine phosphorylation of p190RhoGAP and membrane-protrusive activities at invadopodia." in: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 9-12, (1998) (PubMed).

Settleman, Narasimhan, Foster, Weinberg: "Molecular cloning of cDNAs encoding the GAP-associated protein p190: implications for a signaling pathway from ras to the nucleus." in: Cell, Vol. 69, Issue 3, pp. 539-49, (1992) (PubMed).

Target Details for CNTNAP1

Target: CNTNAP1 (Contactin Associated Protein 1 (CNTNAP1))

Alternative Name: p190

Background: GTPase activating proteins (GAPs) stimulate the GTP-hydrolyzing activity of GTPases, such as p21[ ras]. Ras-GAP (120kDa) is perhaps the best characterized GAP. p190 is a Ras-GAP associated protein that is tyrosine phosphorylated in transformed and growth factor-stimulated cells. Ras-GAP and p190 are targets of oncoproteins and growth factor receptors. Recombinant p190 enhances GTPase activity of the rho family of GTPases. The complete sequence of p190 exhibits three major regions: an N-terminal region with homology to all GTPases, a middle region homologous to transcriptional repressors, and a C-terminal region the is similar to n-chimerin and other small GTPases.

Molecular Weight: 190 kDa

Pathways: Cell-Cell Junction Organization