Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (C-Term) antibody
|Synonyms||uvo, lcam, E-Cad, XBcad, l-cam, xcdh1, cdhc-A, xb-cad, XTCAD-1, E-cadherin, cadherin-1, uvomorulin, XB-cadherin, cdh1|
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
|5 references available|
|Price||Product not available in this region.|
|Immunogen||Human E-Cadherin Recombinant Protein|
|Cross-Reactivity||Dog (Canine), Mouse (Murine), Rat (Rattus)|
E-Cadherin is a 120-kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-cadherin/catenin complexes associate with cortical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression. The 36/E-Cadherin monoclonal antibody recognizes the cytoplasmic domain of E-Cadherin, regardless of phosphorylation status. The peptide immunogen was generated from human E-Cadherin aa. 735-883.
Synonyms: CD324, CDH1, CADH1, Cadherin-1, ECAD, CDHE, Arc-1, LCAM, UVO, Uvomorulin
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||120 kDa|
Related Products: ABIN968533, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Restrictions||For Research Use only|
|Western blot analysis of E-Cadherin on A431 lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of E-Cadherin. Immunofluorescent staining of WIDR cells at 1:50 dilution of E-cadherin.|
Takeichi: "The cadherins: cell-cell adhesion molecules controlling animal morphogenesis." in: Development (Cambridge, England), Vol. 102, Issue 4, pp. 639-55, 1988 (PubMed).
Jaksits, Kriehuber, Charbonnier et al.: "CD34+ cell-derived CD14+ precursor cells develop into Langerhans cells in a TGF-beta 1-dependent manner." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 9, pp. 4869-77, 1999 (PubMed).
Sheibani, Sorenson, Frazier: "Differential modulation of cadherin-mediated cell-cell adhesion by platelet endothelial cell adhesion molecule-1 isoforms through activation of extracellular regulated kinases." in: Molecular biology of the cell, Vol. 11, Issue 8, pp. 2793-802, 2000 (PubMed).
Miyoshi, Shillingford, Smith et al.: "Signal transducer and activator of transcription (Stat) 5 controls the proliferation and differentiation of mammary alveolar epithelium." in: The Journal of cell biology, Vol. 155, Issue 4, pp. 531-42, 2001 (PubMed).
Weng, Xin, Pablo et al.: "Protection against anoikis and down-regulation of cadherin expression by a regulatable beta-catenin protein." in: The Journal of biological chemistry, Vol. 277, Issue 21, pp. 18677-86, 2002 (PubMed).