Fas (TNF Receptor Superfamily, Member 6) (FAS) (AA 1-163) antibody
Alternatives Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
|5 references available|
|Price||Product not available in this region.|
|Cross-Reactivity||Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)|
CD95 is a member of a family of cell surface receptors that includes tumor necrosis factor receptor (TNF-R), nerve growth factor receptor (NGF-R), CD40, CD27, CD30, and 4-1BB. Both murine and human Fas genes have been cloned and reportedly share 60% similarity in their amino acid sequences. CD95 (Fas) is a cell surface apoptosis-signaling molecule that is widely expressed in sites such as thymus, liver, heart, and ovary. Abnormalities in the Fas gene correlate with autoimmune features in mice and with unusually high levels of lymphocyte apoptosis in HIV-infected humans. Genetic studies have localized the Fas gene near the lpr (lymphoproliferation disease) locus on mouse chromosome 19 and further characterization reportedly has revealed that lpr is a mutation affecting the function of the Fas gene. This antibody is routinely tested by western blot analysis.
Synonyms: Fas, APO-1
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||45 kDa|
Related Products: ABIN967389
|Synonyms||APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6, FASL, CD178, CD95L, TNFSF6, APT1LG1, lpr, APO1, TNFR6, Tnfrsf6, AI196731, gld, Fas-L, Faslg, Tnfsf6, Fas-Ligand, Fasl, CD95-L, Apt1Lg1, FASLG, LOC574159, fasl, zgc:162027, FAS|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Research Area||Immunology, Innate Immunity, Cytokines, CD Antigens, Apoptosis/Necrosis, Death Receptors, Cancer, Growth Factors, Surface Receptors of Immune Cells|
|Restrictions||For Research Use only|
|Western blot analysis of CD95 on a Daudi cell lysate (Human B lymphoblast, ATCC CCL-213). Lane 1: 1:5,000, lane 2: 1:10,000, lane 3: 1: 20,000 dilution of the anti- CD95 antibody. Immunohistochemical staining of a formalin-fixed rabbit liver section.|
Itoh, Yonehara, Ishii et al.: "The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis." in: Cell, Vol. 66, Issue 2, pp. 233-43, 1991 (PubMed).
Arnold, Seifert, Asadullah et al.: "Crosstalk between keratinocytes and T lymphocytes via Fas/Fas ligand interaction: modulation by cytokines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 7140-7, 1999 (PubMed).
MacLaren, Clark, Gillespie: "v-Jun sensitizes cells to apoptosis by a mechanism involving mitochondrial cytochrome C release." in: Oncogene, Vol. 19, Issue 51, pp. 5906-18, 2000 (PubMed).
Rosen, Coll, Li et al.: "Transforming growth factor-alpha prevents detachment-induced inhibition of c-Src kinase activity, Bcl-XL down-regulation, and apoptosis of intestinal epithelial cells." in: The Journal of biological chemistry, Vol. 276, Issue 40, pp. 37273-9, 2001 (PubMed).
Zhuang, Demirs, Kochevar: "Protein kinase C inhibits singlet oxygen-induced apoptosis by decreasing caspase-8 activation." in: Oncogene, Vol. 20, Issue 46, pp. 6764-76, 2001 (PubMed).