Ral A (AA 35-206) antibody
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
|5 references available|
|Price||Product not available in this region.|
|Immunogen||Human Ral A|
|Cross-Reactivity||Rat (Rattus), Chicken, Dog (Canine), Mouse (Murine)|
|Description||Ral is a low molecular weight GTP-binding protein belonging to the Ras superfamily of GTP-binding proteins and shows 50% amino acid identity to Ras. Ral cDNA clones have been isolated from human placenta, human pheochromocytoma, simian ß-lymphocyte, and marine ray electric lobe cDNA libraries. In humans, cDNA sequences for Ral A and RalB have been determined. The predicted amino acid sequences show 85% identity. Both proteins consist of 206 amino acids with a predicted molecular weight of 24kDa. However, Ral A isolated from human platelets shows an apparent molecular weight of 26-28kDa. Ral A mRNA levels in adult mouse tissues appear to be highest in testes, ovary, and brain, with lower levels found in the liver, spleen, kidney, thymus, heart, and salivary glands. Similar results have been observed in rat tissues. This antibody is routinely tested by western blot analysis.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols.
|Molecular Weight||24-28 kDa|
Related Products: ABIN968545, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
|Western blot analysis of Ral A on a rat cerebrum lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the anti-Ral A antibody. Immunofluorescence staining of rat neurons at 5 µg/ml of the anti-Ral A antibody.|
Olofsson, Chardin, Touchot et al.: "Expression of the ras-related ralA, rho12 and rab genes in adult mouse tissues." in: Oncogene, Vol. 3, Issue 2, pp. 231-4, 1988 (PubMed).
Polakis, Weber, Nevins et al.: "Identification of the ral and rac1 gene products, low molecular mass GTP-binding proteins from human platelets." in: The Journal of biological chemistry, Vol. 264, Issue 28, pp. 16383-9, 1989 (PubMed).
Goi, Rusanescu, Urano et al.: "Ral-specific guanine nucleotide exchange factor activity opposes other Ras effectors in PC12 cells by inhibiting neurite outgrowth." in: Molecular and cellular biology, Vol. 19, Issue 3, pp. 1731-41, 1999 (PubMed).
Kinashi, Katagiri, Watanabe et al.: "Distinct mechanisms of alpha 5beta 1 integrin activation by Ha-Ras and R-Ras." in: The Journal of biological chemistry, Vol. 275, Issue 29, pp. 22590-6, 2000 (PubMed).
Xu, Frankel, Jackson et al.: "Elevated phospholipase D activity in H-Ras- but not K-Ras-transformed cells by the synergistic action of RalA and ARF6." in: Molecular and cellular biology, Vol. 23, Issue 2, pp. 645-54, 2003 (PubMed).