Ribosomal Protein S6 Kinase Polypeptide 1 (RPS6KA1) (AA 1-184) antibody

Antigen: Ribosomal Protein S6 Kinase Polypeptide 1 (RPS6KA1)

Binding Site: AA 1-184

Clonality: Monoclonal (78)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN967837

Additional Information

Alternative name: Rsk

Immunogen: Human p90[rsk]

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken

Format: Liquid

Isotype: IgG2a

Clone: 78

Description: The p90[rsk] (Rsk) and p70[s6k] kinases were first identified based on their ability to phosphorylate the 40S ribosomal protein S6 in vitro. Both of these enzymes are differentially regulated by serine/threonine phosphorylation in response to mitogenic stimulation. ERK1 and ERK2 have been shown to regulate Rsk activity. Once activated by this phosphorylation, a significant amount of Rsk can be found in the nucleus, suggesting that it has a role in nuclear signaling events. The regulation of nuclear Rsk and ERK activities by growth factors is coordinated with the induction of several early response genes. Rsk has also been shown to be activated by ionizing radiation, presumably through an activated MAP kinase. Studies in Xenopus oocytes and mouse NIH/3T3 cells indicate that inactive Rsk and ERK2 exist in a complex of approximately 110kDa. Upon phosphorylation of Rsk and ERK2, the heterodimer dissociates and at least a portion of these activated kinases translocate to the nucleus.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 90 kDa

Comments:

Related Products: ABIN967389, ABIN968533

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of Rsk on A431 cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Rsk antibody.

Immunofluorescent staining of Hs 766T cells.

Publications

Product: Chung, Pelech, Blenis: "Mitogen-activated Swiss mouse 3T3 RSK kinases I and II are related to pp44mpk from sea star oocytes and participate in the regulation of pp90rsk activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 11, pp. 4981-5, 1991 (PubMed).

Hsiao, Chou, Shih et al.: "Evidence that inactive p42 mitogen-activated protein kinase and inactive Rsk exist as a heterodimer in vivo." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 12, pp. 5480-4, 1994 (PubMed).

Majka, Janowska-Wieczorek, Ratajczak et al.: "Stromal-derived factor 1 and thrombopoietin regulate distinct aspects of human megakaryopoiesis." in: Blood, Vol. 96, Issue 13, pp. 4142-51, 2000 (PubMed).

Moor, Gan, Karmazyn et al.: "Activation of Na+/H+ exchanger-directed protein kinases in the ischemic and ischemic-reperfused rat myocardium." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 16113-22, 2001 (PubMed).

Morrione, Navarro, Romano et al.: "The role of the insulin receptor substrate-1 in the differentiation of rat hippocampal neuronal cells." in: Oncogene, Vol. 20, Issue 35, pp. 4842-52, 2001 (PubMed).