Nitric Oxide Synthase 1, Neuronal (NOS1) (AA 1095-1289) antibody

Details for Product No. ABIN967894
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Antigen
Synonyms nos, nNOS, NOS1, IHPS1, N-NOS, NC-NOS, NOS, bNOS, 2310005C01Rik, NO, NOS-I, Nos-1
Epitope
AA 1095-1289
(23), (9), (7), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(123), (93), (69), (18), (17), (15), (12), (6), (4), (4), (1), (1), (1), (1)
Host
Mouse
(130), (15), (11), (3), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
(107), (55), (36), (32), (31), (30), (26), (20), (10), (9), (1), (1)
Pubmed 5 references available
Quantity 50 µg
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Catalog No. ABIN967894
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Immunogen Human nNOS
Clone Ghr6
Isotype IgG2a
Cross-Reactivity Rat (Rattus), Mouse (Murine), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name nNOS/NOS Type I
Background Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca2+ levels and enhance calmodulin binding. Neuronal NOS (nNOS or bNOS) and endothelial NOS (ECNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin and are regulated in a similar manner. However, both have been shown to be distinct gene products of about 155 kDa and 140 kDa, respectively, and the human forms show 52% amino acid identity. Neuronal NOS and induced macrophage NOS (iNOS) share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain. Neuronal NOS, a cytosolic protein present mainly in neural tissues, has been purified and characterized from rat cerebellum. The NO synthesized by this enzyme acts as a neurotransmitter. ECNOS has been cloned from human vascular endothelium as well as from bovine aortic endothelial cells (BAEC) and has a unique N-myristylation consensus sequence that may explain its membrane localization. This antibody is routinely tested by western blot analysis.
Synonyms: NOS Type I, Neuronal Nitric Oxide Synthase
Molecular Weight 155 kDa
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Nitric Oxide Synthase 1, Neuronal (NOS1) (AA 1095-1289) antibody Immunofluorescence staining on a rabbit brain section.
anti-Nitric Oxide Synthase 1, Neuronal (NOS1) (AA 1095-1289) antibody (2) Western blot analysis of nNOS on a rat pituitary lysate. Lane 1: 1:2500, lane 2: 1: 5000, lane 3: 1: 10,000 dilution of the mouse anti-nNOS antibody.
anti-Nitric Oxide Synthase 1, Neuronal (NOS1) (AA 1095-1289) antibody (3) Immunohistochemical staining of a formalin-fixed paraffin-embedded rat brain tissue section with no pre-treatment (20X magnification).
Product cited in: Nathan: "Nitric oxide as a secretory product of mammalian cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 6, Issue 12, pp. 3051-64, 1992 (PubMed).

Bredt, Hwang, Glatt et al.: "Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase." in: Nature, Vol. 351, Issue 6329, pp. 714-8, 1991 (PubMed).

Yu, Shao, Qian et al.: "Gene transfer of the neuronal NO synthase isoform to cirrhotic rat liver ameliorates portal hypertension." in: The Journal of clinical investigation, Vol. 105, Issue 6, pp. 741-8, 2000 (PubMed).

Sasaki, Gonzalez-Zulueta, Huang et al.: "Dynamic regulation of neuronal NO synthase transcription by calcium influx through a CREB family transcription factor-dependent mechanism." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 15, pp. 8617-22, 2000 (PubMed).

Schuh, Uldrijan, Telkamp et al.: "The plasmamembrane calmodulin-dependent calcium pump: a major regulator of nitric oxide synthase I." in: The Journal of cell biology, Vol. 155, Issue 2, pp. 201-5, 2001 (PubMed).

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