Flotillin 2 (FLOT2) (AA 122-379) antibody

Antigen: Flotillin 2 (FLOT2)

Synonyms: ESA, ECS1, ESA1, ECS-1, M17S1, Esa, AI573412, CG11547, CG14409, FLO2, FLO[Dm]-2, DmelCG32593, CG32593, MGC93331, flot2, MGC52961, FLOT2

Binding Site: AA 122-379

Clonality: Monoclonal (29)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Catalog no.: ABIN967932

Additional Information

Alternative name: Flotillin-2

Immunogen: Human ESA

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken

Format: Liquid

Isotype: IgG1

Clone: 29

Description: Flotillin-2/ESA (epidermal surface antigen) plays a role in intercellular epidermal adhesion. Epidermal surface proteins have an important role in normal epidermal maintenance and function. In addition, they are involved in various autoimmune diseases in which auto-antibodies bind to normal epidermal cell surface proteins. This binding causes cell disadherence that leads to blister formation. Such antibodies have been utilized to identify new proteins involved in epidermal cell adhesion. One of these antibodies, ECS-1, was used to isolate Flotillin-2/ESA. The single copy ESA gene is located on the long arm of human chromosome 17, in the region containing the gene for neurofibromatosis 1 (NF1). ESA mRNA is expressed in cultured keratinocytes, melanocytes, fibroblasts, and carcinoma and melanoma cell lines. Flotillin-2/ESA may play a critical role in epidermal structure and maintenance.
Synonyms: ESA

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 42 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of Flotillin-2 on human endothelial cell lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of anti-Flotillin-2.

Immunofluorescent staining of WI38 cells.

Publications

Product: Schroeder, Stewart-Galetka, Mandavilli et al.: "Cloning and characterization of a novel epidermal cell surface antigen (ESA)." in: The Journal of biological chemistry, Vol. 269, Issue 31, pp. 19983-91, 1994 (PubMed).

Rybin, Xu, Lisanti et al.: "Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway." in: The Journal of biological chemistry, Vol. 275, Issue 52, pp. 41447-57, 2001 (PubMed).

Stuermer, Lang, Kirsch et al.: "Glycosylphosphatidyl inositol-anchored proteins and fyn kinase assemble in noncaveolar plasma membrane microdomains defined by reggie-1 and -2." in: Molecular biology of the cell, Vol. 12, Issue 10, pp. 3031-45, 2001 (PubMed).

Salzer, Hinterdorfer, Hunger et al.: "Ca(++)-dependent vesicle release from erythrocytes involves stomatin-specific lipid rafts, synexin (annexin VII), and sorcin." in: Blood, Vol. 99, Issue 7, pp. 2569-77, 2002 (PubMed).

Nebl, Pestonjamasp, Leszyk et al.: "Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes." in: The Journal of biological chemistry, Vol. 277, Issue 45, pp. 43399-409, 2002 (PubMed).