Vasodilator-Stimulated phosphoprotein (VASP) (AA 248-379) antibody

Antigen: Vasodilator-Stimulated phosphoprotein (VASP)

Synonyms: AA107290, vasp, MGC80889, VASP, MGC127789, wu:fb23b04, wu:fk84g05, zgc:110347, si:dkey-113g17.1, si:ch211-202c21.1

Binding Site: AA 248-379

Clonality: Monoclonal (43)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN967976

Additional Information

Alternative name: VASP

Immunogen: Human VASP - 379

Cross-Reactivity: Dog (Canine)

Format: Liquid

Isotype: IgG1

Clone: 43

Description: Vasodilator-stimulated phosphoprotein (VASP), a substrate for cAMP- and cGMP-dependent kinases, is associated with actin filaments, focal adhesions, and dynamic membrane regions. VASP is composed of several distinct domains: a central L-proline-rich domain contains a GPPPPP motif as a single copy and as a three-fold tandem repeat, as well as three conserved phosphorylation sites for cyclic nucleotide-dependent protein kinases (Ser157, Ser239, and Thr278). A C-terminal domain contains a repetitive mixed-charge cluster which is predicted to form an alpha-helix. The C-terminal domain appears to be responsible for anchoring at focal adhesion sites. VASP has been shown to be a ligand for profilins. Profilins bind to the poly-L-proline motifs of VASP and it is postulated that these two molecules act in concert to convey signal transduction to actin filament formation.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 46 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Signaling, Extracellular Matrix

Restrictions: For Research Use only

Images

Western blot analysis of VASP on human endothelial cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-VASP.

Immunostaining of Human Endothelial cells.

Publications

Product: Reinhard, Giehl, Abel et al.: "The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins." in: The EMBO journal, Vol. 14, Issue 8, pp. 1583-9, 1995 (PubMed).

Haffner, Jarchau, Reinhard et al.: "Molecular cloning, structural analysis and functional expression of the proline-rich focal adhesion and microfilament-associated protein VASP." in: The EMBO journal, Vol. 14, Issue 1, pp. 19-27, 1995 (PubMed).

Lawrence, Pryzwansky: "The vasodilator-stimulated phosphoprotein is regulated by cyclic GMP-dependent protein kinase during neutrophil spreading." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 9, pp. 5550-6, 2001 (PubMed).

Howe, Hogan, Juliano: "Regulation of vasodilator-stimulated phosphoprotein phosphorylation and interaction with Abl by protein kinase A and cell adhesion." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38121-6, 2002 (PubMed).

DeMali, Barlow, Burridge: "Recruitment of the Arp2/3 complex to vinculin: coupling membrane protrusion to matrix adhesion." in: The Journal of cell biology, Vol. 159, Issue 5, pp. 881-91, 2002 (PubMed).