Disabled 2, Mitogen-Responsive Phosphoprotein (DAB2) (AA 31-45) antibody

Antigen: Disabled 2, Mitogen-Responsive Phosphoprotein (DAB2)

Binding Site: AA 31-45

Clonality: Monoclonal (52)

Host: Mouse

Reactivity: Mouse (Murine)

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN967989

Additional Information

Alternative name: p96

Immunogen: Mouse p96

Cross-Reactivity: Human, Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 52

Description: CSF-1 is a growth factor that stimulates the growth and differentiation of immature lymphocytes and is required for the survival of mononuclear phagocytes. Binding of CSF-1 induces dimerization and autophosphorylation of its receptor. This results in the activation of several signal transduction pathways. A unique 96 kDa protein is a component in the CSF-1 signal transduction cascade. p96 is phosphorylated on serine following mitogenic stimulation of a mouse macrophage cell line. p96 contains three potential C-terminal ERK kinase phosphorylation sites, as well as several proline-rich sequences that are potential binding sites for SH3-containing proteins. Structural similarities have been found between p96 and Dab, a product of the Drosophila disabled gene, and p96 was also identified as Disabled-2 (Dab-2) and as differentially expressed in ovarian carcinoma-2 (DOC-2) . Dab-2/p96 has been shown to be essential for TGFbeta signaling by facilitating signal transduction from the TGFbeta receptor to the Smad family of transcription factors. Thus, Dab-2/p96 is an important adaptor molecule in growth factor signaling pathways.
Synonyms: Disabled-2

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 96 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of p96 on BC3H1 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of p96.

Immunofluorescence staining of Human Fibroblast cells.

Publications

Product: Xu, Yang, Jackowski et al.: "Cloning of a novel phosphoprotein regulated by colony-stimulating factor 1 shares a domain with the Drosophila disabled gene product." in: The Journal of biological chemistry, Vol. 270, Issue 23, pp. 14184-91, 1995 (PubMed).

Hocevar, Smine, Xu et al.: "The adaptor molecule Disabled-2 links the transforming growth factor beta receptors to the Smad pathway." in: The EMBO journal, Vol. 20, Issue 11, pp. 2789-801, 2001 (PubMed).

Smith, Capo-chichi, He et al.: "Disabled-2 mediates c-Fos suppression and the cell growth regulatory activity of retinoic acid in embryonic carcinoma cells." in: The Journal of biological chemistry, Vol. 276, Issue 50, pp. 47303-10, 2001 (PubMed).

Mishra, Keyel, Hawryluk et al.: "Disabled-2 exhibits the properties of a cargo-selective endocytic clathrin adaptor." in: The EMBO journal, Vol. 21, Issue 18, pp. 4915-26, 2002 (PubMed).