GLN1 antibody (AA 1-373)

Catalog No. ABIN968015

The Mouse Monoclonal anti-GLN1 antibody has been validated for WB, IHC, IP, IF and BI. It is suitable to detect GLN1 in samples from Human, Mouse and Rat. There are 7+ publications available.

Quick Overview for GLN1 antibody (AA 1-373) (ABIN968015)

Target: GLN1 (Glutamine Synthetase (GLN1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GLN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), BioImaging (BI)

Clone: 6-Glutamine Synthetase

Product Details anti-GLN1 Antibody

Binding Specificity: AA 1-373

Cross-Reactivity: Rat (Rattus), Human, Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Glutamine Synthetase aa. 1-373

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-GLN1 antibody (ABIN968015)

De Sevilla Müller, Liu, Solomon, Rodriguez, Brecha: "Expression of voltage-gated calcium channel α(2)δ(4) subunits in the mouse and rat retina." in: The Journal of comparative neurology, Vol. 521, Issue 11, pp. 2486-501, (2013) (PubMed).

OBrien, Caldwell, Ehring, Bumsted OBrien, Luo, Levinson: "Tetrodotoxin-resistant voltage-gated sodium channels Na(v)1.8 and Na(v)1.9 are expressed in the retina." in: The Journal of comparative neurology, Vol. 508, Issue 6, pp. 940-51, (2008) (PubMed).

Kronfeld, Kazimirsky, Lorenzo, Garfield, Blumberg, Brodie: "Phosphorylation of protein kinase Cdelta on distinct tyrosine residues regulates specific cellular functions." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35491-8, (2000) (PubMed).

Labow, Souba, Abcouwer: "Glutamine synthetase expression in muscle is regulated by transcriptional and posttranscriptional mechanisms." in: The American journal of physiology, Vol. 276, Issue 6 Pt 1, pp. E1136-45, (1999) (PubMed).

Fei, DAmbrosio, Li, Surmacz, Baserga: "Association of insulin receptor substrate 1 with simian virus 40 large T antigen." in: Molecular and cellular biology, Vol. 15, Issue 8, pp. 4232-39, (1995) (PubMed).

Kentroti, Baker, Lee, Bruce, Vernadakis: "Platelet-activating factor increases glutamine synthetase activity in early and late passage C-6 glioma cells." in: Journal of neuroscience research, Vol. 28, Issue 4, pp. 497-506, (1991) (PubMed).

Vardimon, Fox, Cohen-Kupiec, Degenstein, Moscona: "Expression of v-src in embryonic neural retina alters cell adhesion, inhibits histogenesis, and prevents induction of glutamine synthetase." in: Molecular and cellular biology, Vol. 11, Issue 10, pp. 5275-84, (1991) (PubMed).

Target Details for GLN1

Target: GLN1 (Glutamine Synthetase (GLN1))

Alternative Name: Glutamine Synthetase

Background: Glutamine synthetase catalyzes the amination of glutamic acid to form glutamine. It is found in mammals as an octamer of identical 45 kDa subunits. Glutamine synthetase activity is a useful marker for astrocytes and an important differentiation feature in retina. It is also considered to be a key enzyme in the recycling of the neurotransmitter glutamate. This antibody is routinely tested by western blot analysis.

Molecular Weight: 45 kDa

Pathways: Positive Regulation of Peptide Hormone Secretion