TNFRSF1A-Associated Via Death Domain (TRADD) (AA 163-312) antibody

Antigen: TNFRSF1A-Associated Via Death Domain (TRADD)

Synonyms: Hs.89862, MGC11078, MGC103556, wu:fc59e02, zgc:103556, TRADD, MGC137615, tradd

Binding Site: AA 163-312

Clonality: Monoclonal (37)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN968046

Additional Information

Alternative name: TRADD

Immunogen: Human TRADD

Cross-Reactivity: Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 37

Description: TNFalpha (Tumor Necrosis Factor) stimulates programmed cell death and NF-kappaB activation as a result of its binding to the TNF receptor 1 (TNFR1). Within this receptor, a sequence referred to as the death domain” has been shown to be necessary for both of these functions. Using the yeast two-hybrid system to detect proteins which interact with the receptor through this death domain”, a 34 kDa protein was found and designated TRADD (TNFR1-Associated Death Domain protein). TRADD appears to contain no intrinsic catalytic activity. It also contains a death domain and it has been shown to bind to FADD and RIP. Mutational analysis of TRADD demonstrates that programmed cell death and NF-kappaB activation are distinct and controlled independently.
Synonyms: TNFR1-Associated Death Domain protein

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 34 kDa

Comments:

Related Products: ABIN968537, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Cardiovascular, Atherosclerosis, Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of TRADD on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-TRADD antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma, ATCC CCL-2.2).

Publications

Product: Hsu, Xiong, Goeddel: "The TNF receptor 1-associated protein TRADD signals cell death and NF-kappa B activation." in: Cell, Vol. 81, Issue 4, pp. 495-504, 1995 (PubMed).

Park, Baichwal: "Systematic mutational analysis of the death domain of the tumor necrosis factor receptor 1-associated protein TRADD." in: The Journal of biological chemistry, Vol. 271, Issue 16, pp. 9858-62, 1996 (PubMed).

Yount, Afshar, Ries et al.: "Transcriptional activation of TRADD mediates p53-independent radiation-induced apoptosis of glioma cells." in: Oncogene, Vol. 20, Issue 22, pp. 2826-35, 2001 (PubMed).

Morgan, Thorburn, Pandolfi et al.: "Nuclear and cytoplasmic shuttling of TRADD induces apoptosis via different mechanisms." in: The Journal of cell biology, Vol. 157, Issue 6, pp. 975-84, 2002 (PubMed).

He, Ting: "A20 inhibits tumor necrosis factor (TNF) alpha-induced apoptosis by disrupting recruitment of TRADD and RIP to the TNF receptor 1 complex in Jurkat T cells." in: Molecular and cellular biology, Vol. 22, Issue 17, pp. 6034-45, 2002 (PubMed).