NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 244-444) antibody

Antigen: NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2)

Synonyms: NLK1, NEK2A, HsPK21

Binding Site: AA 244-444

Clonality: Monoclonal (20)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Catalog no.: ABIN968053

Additional Information

Alternative name: Nek2

Immunogen: Human Nek2

Format: Liquid

Isotype: IgG1

Clone: 20

Description: Reversible protein phosphorylation is critical for progression through the cell cycle and mitosis. In Aspergillus nidulans, the nima gene (never in mitosis) encodes a protein kinase that is essential for mitosis. Three human genes (nek1, 2, and 3 [Nima-related kinase]) with significant homology to the A. nidulans nima have been reported. The nek2 gene encodes a protein of 445 amino acids and, like its fungal homolog, its expression is regulated throughout the cell cycle. In Hela cells, Nek2 activity and expression are low during M and G1 phases of the cell cycle. However, both parameters increase during S phase and mitosis. In addition, Nek2 phosphorylates protein substrates exclusively at serine and threonine residues. Thus, like its fungal homolog, Nek2 may be a crucial element in controlling the cell's entry into S phase and mitosis. This antibody is routinely tested by western blot analysis.
Synonyms: Nima Related Kinase 2

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 46 kDa

Comments:

Related Products: ABIN968537, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of Nek2 on a Jurkat cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- human Nek2 antibody.

Immunofluorescence staining of rabbit kidney.

Publications

Product: Schultz, Fry, Sütterlin et al.: "Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 5, Issue 6, pp. 625-35, 1994 (PubMed).

Fry, Schultz, Bartek et al.: "Substrate specificity and cell cycle regulation of the Nek2 protein kinase, a potential human homolog of the mitotic regulator NIMA of Aspergillus nidulans." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12899-905, 1995 (PubMed).