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NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 244-444) antibody

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Synonyms AA617254, C77054, HsPK21, NEK2A, NLK1, ATNEK2, NIMA-RELATED KINASE2, NIMA-related kinase 2, T9J14.24, T9J14_24
AA 244-444
(26), (21), (13), (11), (10), (7), (5), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
(126), (26), (24), (1), (1), (1), (1), (1), (1), (1)
(105), (24), (3)
Clonality (Clone)
Monoclonal ()
(9), (9), (7), (6), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(107), (70), (23), (15), (12), (11), (11), (5), (2), (1), (1), (1)
Pubmed 2 references available
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Quantity 50 μg
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Immunogen Human Nek2 aa. 244-444
Clone 20-Nek2
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Nek2 (NEK2 Antibody Abstract)
Background Reversible protein phosphorylation is critical for progression through the cell cycle and mitosis. In Aspergillus nidulans, the nima gene (never in mitosis) encodes a protein kinase that is essential for mitosis. Three human genes (nek1, 2, and 3 [Nima-related kinase]) with significant homology to the A. nidulans nima have been reported. The nek2 gene encodes a protein of 445 amino acids and, like its fungal homolog, its expression is regulated throughout the cell cycle. In Hela cells, Nek2 activity and expression are low during M and G1 phases of the cell cycle. However, both parameters increase during S phase and mitosis. In addition, Nek2 phosphorylates protein substrates exclusively at serine and threonine residues. Thus, like its fungal homolog, Nek2 may be a crucial element in controlling the cell's entry into S phase and mitosis. This antibody is routinely tested by western blot analysis.
Synonyms: Nima Related Kinase 2
Molecular Weight 46 kDa

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 244-444) antibody (ABIN968053) Western blot analysis of Nek2 on a Jurkat cell lysate. Lane 1: 1:250, lane 2: 1:500, ...
Immunofluorescence (IF) image for anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 244-444) antibody (ABIN968053) Immunofluorescence staining of rabbit kidney.
Product cited in: Fry, Schultz, Bartek et al.: "Substrate specificity and cell cycle regulation of the Nek2 protein kinase, a potential human homolog of the mitotic regulator NIMA of Aspergillus nidulans." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12899-905, 1995 (PubMed).

Background publications Schultz, Fry, Sütterlin et al.: "Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 5, Issue 6, pp. 625-35, 1994 (PubMed).

Catalog No. ABIN968053
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