NOS2 antibody (AA 772-787)

Catalog No. ABIN968055

This anti-NOS2 antibody is a Mouse Monoclonal antibody detecting NOS2 in WB, IHC, IP and IF. Suitable for Mouse. This Primary Antibody has been cited in 4+ publications.

Quick Overview for NOS2 antibody (AA 772-787) (ABIN968055)

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NOS2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 2-iNOS

Product Details anti-NOS2 Antibody

Binding Specificity: AA 772-787

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse iNOS aa. 772-787

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968550, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-NOS2 antibody (ABIN968055)

Nathan, Xie: "Regulation of biosynthesis of nitric oxide." in: The Journal of biological chemistry, Vol. 269, Issue 19, pp. 13725-8, (1994) (PubMed).

Koprowski, Zheng, Heber-Katz, Fraser, Rorke, Fu, Hanlon, Dietzschold: "In vivo expression of inducible nitric oxide synthase in experimentally induced neurologic diseases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 7, pp. 3024-7, (1993) (PubMed).

Vodovotz, Bogdan, Paik, Xie, Nathan: "Mechanisms of suppression of macrophage nitric oxide release by transforming growth factor beta." in: The Journal of experimental medicine, Vol. 178, Issue 2, pp. 605-13, (1993) (PubMed).

Xie, Cho, Calaycay, Mumford, Swiderek, Lee, Ding, Troso, Nathan: "Cloning and characterization of inducible nitric oxide synthase from mouse macrophages." in: Science (New York, N.Y.), Vol. 256, Issue 5054, pp. 225-8, (1992) (PubMed).

Target Details for NOS2

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Alternative Name: iNOS/NOS Type II

Background: Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In macrophages and other cell types, NOS (iNOS or macNOS) activity increases following exposure to cytokines (IFN-gamma, TNF-alpha, and IL-1) and microbial products (lipopolysaccharide (LPS)). iNOS isactivated independently of Ca2+/calmodulin and its level of expression is tightly controlled by several transcription factors, including NFkappaB. Data indicates that TGF-ß affects translation of iNOS mRNA and decreases iNOS protein stability. Normally undetectable in brain tissue, iNOS mRNA has been observed in CNS tissues of animals under experimental pathologic conditions. iNOS and nNOS share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain.
Synonyms: NOS Type II

Molecular Weight: 130 kDa

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process