PKA RI alpha (AA 1-381) antibody

Antigen: PKA RI alpha

Binding Site: AA 1-381

Clonality: Monoclonal (20)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN968061

Additional Information

Immunogen: Human PKA [RIalpha]

Cross-Reactivity: Rat (Rattus), Dog (Canine), Mouse (Murine), Chicken

Format: Liquid

Isotype: IgG1

Clone: 20

Description: CAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have been identified: RIalpha, RIbeta, RIIalpha, and RIIbeta. These subunits define type I and II cAMP-dependent protein kinases. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Calpha, Cbeta, or Cgamma). Type II PKA can be distinguished by autophosphorylation of the R-subunits, while type I PKA binds Mg/ATP with high affinity. Most cells express both type I and type II PKAs. Although the Ralpha isoforms are ubiquitously expressed, the Rbeta isoforms are predominant in nervous and adipose tissues. Expression of the RIbeta subunit is modulated during muscle and adipocyte differentiation in vitro.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 49 kDa

Comments:

Related Products: ABIN968545, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of PKA [RIalpha] on a rat cerebrum lysate. Lane 1, 1:250, lane 2, 1:500, lane 3: 1:1000 dilution of the mouse anti-PKA [RIalpha] antibody.

Publications

Product: Skålhegg, Landmark, Foss et al.: "Identification, purification, and characterization of subunits of cAMP-dependent protein kinase in human testis. Reverse mobilities of human RII alpha and RII beta on sodium dodecyl sulfate-polyacrylamide gel electrophoresis compared with rat and bovine R" in: The Journal of biological chemistry, Vol. 267, Issue 8, pp. 5374-9, 1992 (PubMed).

Sandberg, Skålhegg, Jahnsen: "The two mRNA forms for the type I alpha regulatory subunit of cAMP-dependent protein kinase from human testis are due to the use of different polyadenylation site signals." in: Biochemical and biophysical research communications, Vol. 167, Issue 1, pp. 323-30, 1990 (PubMed).

Casey, Vaughan, He et al.: "Mutations in the protein kinase A R1alpha regulatory subunit cause familial cardiac myxomas and Carney complex." in: The Journal of clinical investigation, Vol. 106, Issue 5, pp. R31-8, 2000 (PubMed).

Vang, Torgersen, Sundvold et al.: "Activation of the COOH-terminal Src kinase (Csk) by cAMP-dependent protein kinase inhibits signaling through the T cell receptor." in: The Journal of experimental medicine, Vol. 193, Issue 4, pp. 497-507, 2001 (PubMed).

Taskén, Collas, Kemmner et al.: "Phosphodiesterase 4D and protein kinase a type II constitute a signaling unit in the centrosomal area." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 21999-2002, 2001 (PubMed).