PKA RI alpha (AA 1-381) antibody

Details for Product No. ABIN968061
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Antigen
Epitope
AA 1-381
(1)
Reactivity
Human
(1)
Host
Mouse
(1)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
(1)
Pubmed 5 references available
Quantity 50 μg
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Immunogen Human PKA [RIalpha]
Clone LEP-02
Isotype IgG1
Cross-Reactivity Rat (Rattus), Dog (Canine), Mouse (Murine), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Background CAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have been identified: RIalpha, RIbeta, RIIalpha, and RIIbeta. These subunits define type I and II cAMP-dependent protein kinases. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Calpha, Cbeta, or Cgamma). Type II PKA can be distinguished by autophosphorylation of the R-subunits, while type I PKA binds Mg/ATP with high affinity. Most cells express both type I and type II PKAs. Although the Ralpha isoforms are ubiquitously expressed, the Rbeta isoforms are predominant in nervous and adipose tissues. Expression of the RIbeta subunit is modulated during muscle and adipocyte differentiation in vitro.
Molecular Weight 49 kDa
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-PKA RI alpha (AA 1-381) antibody Western blot analysis of PKA [RIalpha] on a rat cerebrum lysate. Lane 1, 1:250, lane ...
Product cited in: Taskén, Collas, Kemmner et al.: "Phosphodiesterase 4D and protein kinase a type II constitute a signaling unit in the centrosomal area." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 21999-2002, 2001 (PubMed).

Vang, Torgersen, Sundvold et al.: "Activation of the COOH-terminal Src kinase (Csk) by cAMP-dependent protein kinase inhibits signaling through the T cell receptor." in: The Journal of experimental medicine, Vol. 193, Issue 4, pp. 497-507, 2001 (PubMed).

Casey, Vaughan, He et al.: "Mutations in the protein kinase A R1alpha regulatory subunit cause familial cardiac myxomas and Carney complex." in: The Journal of clinical investigation, Vol. 106, Issue 5, pp. R31-8, 2000 (PubMed).

Sandberg, Skålhegg, Jahnsen: "The two mRNA forms for the type I alpha regulatory subunit of cAMP-dependent protein kinase from human testis are due to the use of different polyadenylation site signals." in: Biochemical and biophysical research communications, Vol. 167, Issue 1, pp. 323-30, 1990 (PubMed).

Skålhegg, Landmark, Foss et al.: "Identification, purification, and characterization of subunits of cAMP-dependent protein kinase in human testis. Reverse mobilities of human RII alpha and RII beta on sodium dodecyl sulfate-polyacrylamide gel electrophoresis compared with rat and bovine R" in: The Journal of biological chemistry, Vol. 267, Issue 8, pp. 5374-9, 1992 (PubMed).

Validation Images
Catalog No. ABIN968061
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