Syntaxin 6 (STX6) (AA 6-136) antibody

Antigen: Syntaxin 6 (STX6)

Synonyms: MGC93348, CT23545, Dm Syx6, DmSyx6, SYX6/10, Syx, DmelCG7736, CG7736, AA437559, AI415714, AI845861, 2310039E05Rik, 2410005I16Rik, MGC78971, MGC132090, STX6, MGC151807, fb51b08, wu:fb51b08, zgc:110658, si:dkey-121a11.2, LOC734102, Syx6, Bmsyx6, Syntaxin-6, DKFZp459N181

Binding Site: AA 6-136

Clonality: Monoclonal (30)

Host: Mouse

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN968080

Additional Information

Alternative name: Syntaxin 6

Immunogen: Rat Syntaxin 6

Cross-Reactivity: Human, Mouse (Murine), Dog (Canine), Chicken, Frog

Format: Liquid

Isotype: IgG1

Clone: 30

Description: Signal transmission between cells is often regulated by the secretion of neurotransmitters or hormones into the extracellular space. This process is controlled by a complex pathway of membrane trafficking which leads to membrane fusion and secretion. Syntaxin 1 is involved in three important complexes that modulate the secretion process: syntaxin and n-sec-1, syntaxin, VAMP, and SNAP-25, and syntaxin, VAMP, SNAP-25, alphaSNAP, and NSF (20S complex). Syntaxin 6 is a 255 amino acid member of the syntaxin family. It contains a C-terminal transmembrane domain and is located at the Golgi apparatus. In addition, Syntaxin 6 displays significant (47% identity) homology with SNAP-25. In vitro, Syntaxin 6 has been reported to interact with alpha-SNAP. Syntaxin 6 has been reported to have a calculated molecular weight of 30.6 kDa, but has been observed to migrate in a range inbetween ~ 31-35 kDa.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 31-35 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Synonyms: MGC93348, CT23545, Dm Syx6, DmSyx6, SYX6/10, Syx, DmelCG7736, CG7736, AA437559, AI415714, AI845861, 2310039E05Rik, 2410005I16Rik, MGC78971, MGC132090, STX6, MGC151807, fb51b08, wu:fb51b08, zgc:110658, si:dkey-121a11.2, LOC734102, Syx6, Bmsyx6, Syntaxin-6, DKFZp459N181

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Signaling

Restrictions: For Research Use only

Images

Western blot analysis of Syntaxin 6 on a A431 cell lysate (Human epithelial carcinoma, ATCC CRL-1555). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-Syntaxin 6 antibody.

Immunofluorescence staining of PSFK-1 cells (Human neuroectodermal tumor line, ATCC CRL-2060).

Immunohistochemical staining on a rat brain section for Syntaxin 6. A formalin-fixed paraffin-embedded section with citrate buffer pretreatment (40X magnification).

Publications

Product: Bock, Lin, Scheller: "A new syntaxin family member implicated in targeting of intracellular transport vesicles." in: The Journal of biological chemistry, Vol. 271, Issue 30, pp. 17961-5, 1996 (PubMed).

Ramalho-Santos, Moreno, Sutovsky et al.: "SNAREs in mammalian sperm: possible implications for fertilization." in: Developmental biology, Vol. 223, Issue 1, pp. 54-69, 2000 (PubMed).

Mullock, Smith, Ihrke et al.: "Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and Is required for late endosome-lysosome fusion." in: Molecular biology of the cell, Vol. 11, Issue 9, pp. 3137-53, 2000 (PubMed).

Shewan, van Dam, Martin et al.: "GLUT4 recycles via a trans-Golgi network (TGN) subdomain enriched in Syntaxins 6 and 16 but not TGN38: involvement of an acidic targeting motif." in: Molecular biology of the cell, Vol. 14, Issue 3, pp. 973-86, 2003 (PubMed).