Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Syntaxin 6 antibody

STX6 Reactivity: Mouse, Rat, Hamster WB, IF, IP Host: Mouse Monoclonal 3D10 unconjugated
Catalog No. ABIN492572
  • Target See all Syntaxin 6 (STX6) Antibodies
    Syntaxin 6 (STX6)
    Reactivity
    • 62
    • 17
    • 16
    • 10
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse, Rat, Hamster
    Host
    • 63
    • 5
    • 2
    • 2
    Mouse
    Clonality
    • 67
    • 5
    Monoclonal
    Conjugate
    • 26
    • 8
    • 8
    • 6
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Syntaxin 6 antibody is un-conjugated
    Application
    • 51
    • 34
    • 13
    • 13
    • 8
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
    Specificity
    This antibody reacts with 31 kDa membrane protein Syntaxin-6 on Western blotting and Immunoprecipitation. It is reported that this monoclonal antibody (3D10) binds to the amino-terminal 25 amino acid of Rat Syntaxin-6 (Ref.6). Detects a band of approximately 35 kDa (predicted molecular weight: 30.6 kDa).
    Cross-Reactivity (Details)
    Species reactivity (tested):Mouse, Hamster and Rat.It is reported that this clone 3D10 reacted with Human Syntaxin-6 in Reference 3.
    Characteristics
    Synonyms: Syntaxin-6, Golgi Marker
    Purification
    Protein-A Sepharose Chromatography of hybridoma supernatant.
    Immunogen
    Recombinant Rat Syntaxin-6. Remarks: Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/cmouse splenocyte
    Clone
    3D10
    Isotype
    IgG1
    Top Product
    Discover our top product STX6 Primary Antibody
  • Application Notes
    Western blotting: 1 μg/mL for chemiluminescence detection system. Immunoprecipitation: 2 μg/300 μL of cell extract. Immunocytochemistry. Detailed procedure is provided in Protocols.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocol
    SDS-PAGE & Western Blotting1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10%glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. Measure the protein concentration of the supernatant and add the cold Lysisbuffer to make 8 mg/mL solution. 3) Mix the sample with equal volume of Laemmli’s sample buffer. 4) Boil the samples for 3 minutes and centrifuge. Load 10 μL of the sample per lane in a 1mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm2 for 1 hourin a semi-dry transfer system (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20% MeOH). See the manufacture's manual for precise transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10% skimmed milk (in PBS, pH7. 2) for 1 hour at room temperature, or overnight at 4°C. 7) Incubate the membrane with primary antibody diluted with PBS, pH 7. 2 containing 1%skimmed milk as suggest in the APPLICATIONS for 1 hour at room temperature. (Theconcentration of antibody will depend on condition. )8) Wash the membrane with PBS-T [0. 05% Tween-20 in PBS] (5 minutes x 3 times). 9) Incubate the membrane with the 1: 10,000 HRP-conjugated anti-mouse IgG diluted with1% skimmed milk (in PBS, pH 7. 2) for 1 hour at room temperature. 10) Wash the membrane with PBS-T (10 minutes x 3 times). 11) Wipe excess buffer on the membrane, then incubate it with appropriatechemiluminescence reagent for 1 minute. 12) Remove extra reagent from the membrane by dabbing with paper towel, and seal it inplastic wrap. 13) Expose to an X-ray film in a dark room for 3 minutes. 14) Develop the film as usual. The condition for exposure and development may vary. Positive Controls: Rat brain, WR19LImmunoprecipitation1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, pH 7.2 containing 50 % Glycerol without preservatives.
    Preservative
    Without preservative
    Storage
    -20 °C
    Storage Comment
    Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    Syntaxin 6 (STX6)
    Alternative Name
    Syntaxin 6 / STX6 (STX6 Products)
    Synonyms
    CG7736 antibody, CT23545 antibody, Dm Syx6 antibody, DmSyx6 antibody, Dmel\\CG7736 antibody, SYN6 antibody, SYX6/10 antibody, Syx antibody, MGC78971 antibody, MGC132090 antibody, STX6 antibody, fb51b08 antibody, wu:fb51b08 antibody, zgc:110658 antibody, si:dkey-121a11.2 antibody, LOC734102 antibody, Syx6 antibody, Bmsyx6 antibody, Syntaxin-6 antibody, 2310039E05Rik antibody, 2410005I16Rik antibody, AA437559 antibody, AI415714 antibody, AI845861 antibody, Syntaxin 6 antibody, syntaxin 6 L homeolog antibody, syntaxin 6 antibody, Syx6 antibody, stx6.L antibody, STX6 antibody, stx6 antibody, Stx6 antibody
    Background
    Key requirements for protein transport are vesicular carriers with a full complement of machinery to enable them to find and fuse with the correct downstream compartment. This machinery includes the soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein (SNAP) receptors (SNAREs). SNAREs mediate diverse membrane fusion events such as neurotransmitter-filled vesicles fusing with the presynaptic plasma membrane. Syntaxin-6 is a q-SNARE found in endosomal transport vesicles. Syntaxin-6 has been shown by electron microscopy to localize mostly to the trans-Golgi network (TGN) and, to a lesser extent, to the Golgi stack.Synonyms: Golgi Marker, Syntaxin-6
    Gene ID
    58244
    UniProt
    Q9JKK1
    Pathways
    Synaptic Vesicle Exocytosis
You are here:
Support