SNRPN antibody (AA 14-174)

Catalog No. ABIN968085

This anti-SNRPN antibody is a Mouse Monoclonal antibody detecting SNRPN in WB, IF, BI and IHC (f). Suitable for Human, Mouse, Rat and Dog. This Primary Antibody has been cited in 5+ publications.

Quick Overview for SNRPN antibody (AA 14-174) (ABIN968085)

Target: SNRPN (Small Nuclear Ribonucleoprotein Polypeptide N (SNRPN))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This SNRPN antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 8-SMN

Product Details anti-SNRPN Antibody

Binding Specificity: AA 14-174

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Alexa Fluor is a registered trademark of Molecular Probes, Inc., Eugene, OR.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human SMN aa. 14-174

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968587, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-SNRPN antibody (ABIN968085)

Briese, Richter, Sattelle, Ulfig: "SMN, the product of the spinal muscular atrophy-determining gene, is expressed widely but selectively in the developing human forebrain." in: The Journal of comparative neurology, Vol. 497, Issue 5, pp. 808-16, (2006) (PubMed).

Côté, Boisvert, Boulanger, Bedford, Richard: "Sam68 RNA binding protein is an in vivo substrate for protein arginine N-methyltransferase 1." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 274-87, (2003) (PubMed).

Wang, Reddy, Shen: "Higher order arrangement of the eukaryotic nuclear bodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 21, pp. 13583-8, (2002) (PubMed).

Claus, Doring, Gringel, Muller-Ostermeyer, Fuhlrott, Kraft, Grothe: "Differential intranuclear localization of fibroblast growth factor-2 isoforms and specific interaction with the survival of motoneuron protein." in: The Journal of biological chemistry, Vol. 278, Issue 1, pp. 479-85, (2002) (PubMed).

Cifuentes-Diaz, Frugier, Tiziano, Lacène, Roblot, Joshi, Moreau, Melki: "Deletion of murine SMN exon 7 directed to skeletal muscle leads to severe muscular dystrophy." in: The Journal of cell biology, Vol. 152, Issue 5, pp. 1107-14, (2001) (PubMed).

Target Details for SNRPN

Target: SNRPN (Small Nuclear Ribonucleoprotein Polypeptide N (SNRPN))

Alternative Name: SMN

Background: SMN (survival motor neuron) was discovered as a candidate gene, located in chromosome 5q13, for the fatal autosomal Spinal muscular atrophy (SMA) disorder. The SMN gene was missing or interrupted in a significant number of patients with SMA. The SMN protein is 294 amino acids and migrates with apparent molecular weight of 40 kDa. In addition to the cytoplasm, other studies localized SMN in dots of 0.1-1.0 µm within the nucleus. These novel nuclear structures were named gems and found associated to coiled bodies. It was also found that SMN interacts with the RGG box of hnRNP U and fibrillarin. Therefore, the biochemical function of SMN may be in the regulation of mRNA metabolism.
Synonyms: Survival Motor Neuron

Molecular Weight: 40 kDa