N-Acetyltransferase 1 (Arylamine N-Acetyltransferase) (NAT1) (AA 672-830) antibody

Antigen: N-Acetyltransferase 1 (Arylamine N-Acetyltransferase) (NAT1)

Synonyms: AAC1, NATI, Nat-1, NAT1

Binding Site: AA 672-830

Clonality: Monoclonal (35)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968149

Additional Information

Alternative name: NAT1

Immunogen: Human NAT1

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)

Format: Liquid

Isotype: IgG1

Clone: 35

Description: EIF-4 proteins are required for recognition of mRNA and acceleration of protein translation. This group of proteins consists of the RNA helicase eIF-4A, the RNA-binding protein eIF-4B, the cap-binding protein eIF-4E, and eIF-4G (p220). NAT1 (Novel APOBEC-1 Target no. 1), also known as DAP-5 (Death Associated Protein -5), is homologous to eIF-4G. Amino acid sequence comparison of NAT1 and eIF-4 shows that NAT1 lacks an eIF-4G N-terminal region. This region mediates eIF-4G association with eIF-4E. The highest degree of homology is within the central portions of NAT1 and eIF-4G, while the lowest degree of homology occurs at the C-terminus. NAT1 more closely resembles a cleaved form of eIF-4G that is involved in cap-independent translation. It is thought that NAT1 is involved in the repression of translation via its inhibition of both cap-dependent and cap-independent translation.
Synonyms: Novel APOBEC-1 Target no. 1, DAP-5, Death Associated Protein -5

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 100 kDa

Comments:

Related Products: ABIN968535, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Signaling, Metabolism, Amino Acids

Restrictions: For Research Use only

Images

Western blot analysis of NAT1 on a HeLa cell lysate (Human cervical epitheloid carcinoma, ATCC CCL-2). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-NAT1 antibody.

Immunofluorescence staining of mouse macrophages.

Publications

Product: Yamanaka, Poksay, Arnold et al.: "A novel translational repressor mRNA is edited extensively in livers containing tumors caused by the transgene expression of the apoB mRNA-editing enzyme." in: Genes & development, Vol. 11, Issue 3, pp. 321-33, 1997 (PubMed).

Levy-Strumpf, Deiss, Berissi et al.: "DAP-5, a novel homolog of eukaryotic translation initiation factor 4G isolated as a putative modulator of gamma interferon-induced programmed cell death." in: Molecular and cellular biology, Vol. 17, Issue 3, pp. 1615-25, 1997 (PubMed).

Henis-Korenblit, Strumpf, Goldstaub et al.: "A novel form of DAP5 protein accumulates in apoptotic cells as a result of caspase cleavage and internal ribosome entry site-mediated translation." in: Molecular and cellular biology, Vol. 20, Issue 2, pp. 496-506, 2000 (PubMed).