RON alpha (AA 40-224) antibody

Antigen: RON alpha

Binding Site: AA 40-224

Clonality: Monoclonal (29)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968151

Additional Information

Immunogen: Human RON alpha

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Frog

Format: Liquid

Isotype: IgG2b

Clone: 29

Description: RON (Recepteur d'Origine Nantaise) is a tyrosine kinase receptor for MSP (Macrophage Stimulating Protein) that belongs to the hepatocyte growth factor receptor family. RON is synthesized as a single chain glycosylated polypeptide of 1400 amino acids that is cleaved upon maturation into a 150 kDa beta-subunit with the kinase activity and a 40 kDa alpha-chain. Both chains are linked by disulfide bonds and the proper arrangement of the alpha and beta heterodimer is necessary for biological activity of RON. MSP induces the tyrosine kinase activity of RON, triggering a cascade of intracellular signaling that leads to DNA synthesis. However, RON activation in mouse erythroleukemia cells results in apoptosis. The replacement of autophosphorylation sites Y1330 and Y1337 abolishes both the mitogenic and apoptotic effects of MSP. Therefore, autophosphorylation at Y1330 and Y1337 is required for RON's biological activity. PLCgamma, Shc, Grb2, p61, and p65 associate with RON. Although widely expressed, RON is particularly abundant in epithelial tissues, lung, brain, adrenal glands, gastrointestinal tract, kidney, and testis.
Synonyms: Recepteur d'Origine Nantaise

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 40 kDa

Comments:

Related Products: ABIN968619, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of RONalpha on a MDCK cell lysate (Canine kidney, ATCC CCL-34). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-RONalpha antibody.

Immunofluorescence staining of HS 766T cells (Human pancreatic carcinoma, ATCC HTB-134).

Publications

Product: Wang, Ronsin, Gesnel et al.: "Identification of the ron gene product as the receptor for the human macrophage stimulating protein." in: Science (New York, N.Y.), Vol. 266, Issue 5182, pp. 117-9, 1994 (PubMed).

Gaudino, Follenzi, Naldini et al.: "RON is a heterodimeric tyrosine kinase receptor activated by the HGF homologue MSP." in: The EMBO journal, Vol. 13, Issue 15, pp. 3524-32, 1994 (PubMed).

Ronsin, Muscatelli, Mattei et al.: "A novel putative receptor protein tyrosine kinase of the met family." in: Oncogene, Vol. 8, Issue 5, pp. 1195-202, 1993 (PubMed).

Peace, Hughes, Degen et al.: "Point mutations and overexpression of Ron induce transformation, tumor formation, and metastasis." in: Oncogene, Vol. 20, Issue 43, pp. 6142-51, 2001 (PubMed).