Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 26-156) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2I (UBE2I)

Synonyms: P18, UBC9, C358B7.1, ubc9, zUbc9, Ubce9, Ubce2i, 5830467E05Rik, F830028O17Rik, UBE2I

Binding Site: AA 26-156

Clonality: Monoclonal (50)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN968155

Additional Information

Alternative name: Ubc9

Immunogen: Human Ubc9

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken

Format: Liquid

Isotype: IgG2a

Clone: 50

Description: Progression of the mammalian cell cycle is primarily regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. Ubiquitin, a soluble protein of 76 amino acids, is enzymatically attached to an e-NH2-Lys in a target protein. Ubiquitination is a hallmark for rapid protein degradation of the target protein in the proteosome (a cytoplasmic complex of proteases). Human homologs of the yeast ubiquitin-conjugating enzymes (Ubc) have been reported, including Ubc9. Ubc9 is 158 amino acids with an apparent molecular weight of 18kDa. Although ubiquitously expressed, the highest levels of Ubc9 are found in testis and thymus. Ubc9 was localized to the synaptonemal complex in male mouse sex chromosomes. Furthermore, Ubc9 interacts with the recombination protein Rad51, thus suggesting an important role for Ubc9 during meiosis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 18 kDa

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of Ubc9 on human endothelial lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of Ubc9.

Human Endothelial

Publications

Product: Kovalenko, Plug, Haaf et al.: "Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 7, pp. 2958-63, 1996 (PubMed).

Wang, Qiu, Seufert et al.: "Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 24811-6, 1996 (PubMed).

Buschmann, Lerner, Lee et al.: "The Mdm-2 amino terminus is required for Mdm2 binding and SUMO-1 conjugation by the E2 SUMO-1 conjugating enzyme Ubc9." in: The Journal of biological chemistry, Vol. 276, Issue 44, pp. 40389-95, 2001 (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, 2002 (PubMed).