BMX Non-Receptor Tyrosine Kinase (BMX) (AA 138-276) antibody

Antigen: BMX Non-Receptor Tyrosine Kinase (BMX)

Synonyms: ETK, PSCTK2, PSCTK3, Etk, Tyro8, Etk/Bmx, RGD1565643, BMX

Binding Site: AA 138-276

Clonality: Monoclonal (40)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968180

Additional Information

Alternative name: BMX

Immunogen: Human BMX

Cross-Reactivity: Mouse (Murine), Chicken

Format: Liquid

Isotype: IgG1

Clone: 40

Description: BMX (bone marrow X kinase) is a cytoplasmic tyrosine kinase identified by reverse transcription of mRNA isolated from human bone marrow and mapped to the chromosomal band Xp22.2. The full length protein is 675 amino acids with a tyrosine kinase domain, an amino terminal pleckstrin domain, as well as an SH3 and SH2 domain. Direct comparison of BMX's primary sequence with other kinases showed that this is highly related to the family of BTK/ITK/TEC. BMX kinase is expressed in fetal and adult tissues, with the highest expression in heart, testis, small intestine and colon. It is undetectable in spleen, brain, kidney, and pancreas. Further analysis of mRNA expression showed that BMX is expressed in hematopoietic tissues and neutrophilic granulocytes, and in patients with acute and myeloid leukemia. The levels of BMX mRNA were substantially lower in patients with acute and chronic lymphoid leukemias, thus suggesting that BMX may be important during myelopoiesis. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 80 kDa

Comments:

Related Products: ABIN968537, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Research Area: Signaling, Tyrosine Kinases

Restrictions: For Research Use only

Images

Western blot analysis of BMX on Jurkat lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-BMX.

Publications

Product: Tamagnone, Lahtinen, Mustonen et al.: "BMX, a novel nonreceptor tyrosine kinase gene of the BTK/ITK/TEC/TXK family located in chromosome Xp22.2." in: Oncogene, Vol. 9, Issue 12, pp. 3683-8, 1994 (PubMed).

Kaukonen, Lahtinen, Laine et al.: "BMX tyrosine kinase gene is expressed in granulocytes and myeloid leukaemias." in: British journal of haematology, Vol. 94, Issue 3, pp. 455-60, 1996 (PubMed).

Rajantie, Ekman, Iljin et al.: "Bmx tyrosine kinase has a redundant function downstream of angiopoietin and vascular endothelial growth factor receptors in arterial endothelium." in: Molecular and cellular biology, Vol. 21, Issue 14, pp. 4647-55, 2001 (PubMed).

Vargas, Nore, Berglof et al.: "Functional interaction of caveolin-1 with Bruton's tyrosine kinase and Bmx." in: The Journal of biological chemistry, Vol. 277, Issue 11, pp. 9351-7, 2002 (PubMed).