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Flotillin 1 antibody (AA 312-428)

FLOT1 Reactivity: Human, Mouse, Rat, Chicken WB, IF Host: Mouse Monoclonal 18-Flotillin unconjugated
Catalog No. ABIN968194
  • Target See all Flotillin 1 (FLOT1) Antibodies
    Flotillin 1 (FLOT1)
    Binding Specificity
    • 8
    • 7
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 312-428
    Reactivity
    • 58
    • 26
    • 25
    • 7
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Chicken
    Host
    • 56
    • 2
    • 1
    Mouse
    Clonality
    • 48
    • 11
    Monoclonal
    Conjugate
    • 28
    • 7
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This Flotillin 1 antibody is un-conjugated
    Application
    • 37
    • 23
    • 10
    • 7
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Cross-Reactivity
    Rat (Rattus), Chicken, Human
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Mouse Flotillin aa. 312-428
    Clone
    18-Flotillin
    Isotype
    IgG1
    Top Product
    Discover our top product FLOT1 Primary Antibody
  • Comment

    Related Products: ABIN968545

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20°C.
  • Yamamori, Itakura, Sugaya, Katsumata, Sakagami, Takahashi: "Differential expression of SNAP-25 family proteins in the mouse brain." in: The Journal of comparative neurology, Vol. 519, Issue 5, pp. 916-32, (2011) (PubMed).

    Cabin, Shimazu, Murphy, Cole, Gottschalk, McIlwain, Orrison, Chen, Ellis, Paylor, Lu, Nussbaum: "Synaptic vesicle depletion correlates with attenuated synaptic responses to prolonged repetitive stimulation in mice lacking alpha-synuclein." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 20, pp. 8797-807, (2002) (PubMed).

    Fallon, Moreau, Croft, Labib, Gu, Fon: "Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 486-91, (2002) (PubMed).

    Morrow, Rea, Martin, Prior, Prohaska, Hancock, James, Parton: "Flotillin-1/reggie-2 traffics to surface raft domains via a novel golgi-independent pathway. Identification of a novel membrane targeting domain and a role for palmitoylation." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48834-41, (2002) (PubMed).

    Rybin, Xu, Lisanti, Steinberg: "Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway." in: The Journal of biological chemistry, Vol. 275, Issue 52, pp. 41447-57, (2001) (PubMed).

  • Target
    Flotillin 1 (FLOT1)
    Alternative Name
    Flotillin-1 (FLOT1 Products)
    Synonyms
    reggie-2 antibody, flot1 antibody, Rareg antibody, flot1c antibody, flotillin-1 antibody, flotillin1 antibody, flot1a antibody, reggie2 antibody, si:dz44o19.1 antibody, wu:fj45g11 antibody, flotillin 1 antibody, flotillin-1 antibody, putative flotillin-1 antibody, flotillin 1 S homeolog antibody, flotillin 1 L homeolog antibody, flotillin 1a antibody, FLOT1 antibody, Flot1 antibody, flot1 antibody, LOC5566722 antibody, CpipJ_CPIJ008623 antibody, Smp_016200.1 antibody, flot1.S antibody, flot1.L antibody, flot1a antibody
    Background
    Caveolae are specialized membrane invaginations of 50-100 nm present in all cells, but abundant in endothelium, muscle cells, and adipocytes. These plasma membrane microdomains function in transcytosis of macromolecules, and are the sites of potocytosis, where small molecules are concentrated and transferred inside the cells by glycosylphosphatidylinositol (GPI)-linked receptors. Caveolin, a 22kDa protein and a well known marker for these plasma membrane microdomains, plays a structural role in these specializations. Flotillin-1 was isolated from the Triton X-100 insoluble buoyant fraction, characteristic of caveolae. Although the mRNA expression of both Flotillin-1 and Caveolin is very similar, Caveolin is undetectable in brain, while Flotillin-1 is very abundant. Flotillin-1 is a close homolog of the Epidermal Surface Antigen (ESA/Flotillin-2), which also colocalizes in the caveolae. Thus, Flotillin-1 and its relative ESA/Flotillin-2 are now incorporated into the expanding list of proteins co-localized at the caveolae which includes PKCalpha, Ras, Rap Src-like kinases, Galphaßgamma,and GPI-linked receptors. This antibody is routinely tested by western blot analysis.
    Molecular Weight
    48 kDa
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