Flotillin 1 (FLOT1) (AA 312-428) antibody

Details for Product No. ABIN968194
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Antigen
Synonyms reggie-2, Rareg, flot1, reggie2, si:dz44o19.1, wu:fj45g11, flot1c, flotillin-1, flotillin1, flot1a
Epitope
AA 312-428
(6), (2), (2), (1), (1), (1)
Reactivity
Mouse (Murine)
(30), (19), (15), (14), (13), (13), (1)
Host
Mouse
(29), (6), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(26), (9), (9), (4), (3), (2), (1)
Pubmed 6 references available
Catalog no. ABIN968194
Quantity 50 µg
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Immunogen Mouse Flotillin
Clone 18
Isotype IgG1
Cross-Reactivity Rat (Rattus), Chicken, Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Flotillin-1
Background Caveolae are specialized membrane invaginations of 50-100 nm present in all cells, but abundant in endothelium, muscle cells, and adipocytes. These plasma membrane microdomains function in transcytosis of macromolecules, and are the sites of potocytosis, where small molecules are concentrated and transferred inside the cells by glycosylphosphatidylinositol (GPI)-linked receptors. Caveolin, a 22kDa protein and a well known marker for these plasma membrane microdomains, plays a structural role in these specializations. Flotillin-1 was isolated from the Triton X-100 insoluble buoyant fraction, characteristic of caveolae. Although the mRNA expression of both Flotillin-1 and Caveolin is very similar, Caveolin is undetectable in brain, while Flotillin-1 is very abundant. Flotillin-1 is a close homolog of the Epidermal Surface Antigen (ESA/Flotillin-2), which also colocalizes in the caveolae. Thus, Flotillin-1 and its relative ESA/Flotillin-2 are now incorporated into the expanding list of proteins co-localized at the caveolae which includes PKCalpha, Ras, Rap Src-like kinases, Galphaßgamma,and GPI-linked receptors. This antibody is routinely tested by western blot analysis.
Molecular Weight 48 kDa
Comment

Related Products: ABIN968545

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Supplier Images
anti-Flotillin 1 (FLOT1) (AA 312-428) antibody Western blot analysis of flotillin-1 on rat brain lysate. Lane 1: 1:250, Lane 2: 1:500, Lane 3: 1:1000 dilution of flotillin-1.
Product cited in: Bickel, Scherer, Schnitzer et al.: "Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins." in: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13793-802, 1997 (PubMed).

Rybin, Xu, Lisanti et al.: "Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway." in: The Journal of biological chemistry, Vol. 275, Issue 52, pp. 41447-57, 2001 (PubMed).

Fallon, Moreau, Croft et al.: "Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 486-91, 2002 (PubMed).

Morrow, Rea, Martin et al.: "Flotillin-1/reggie-2 traffics to surface raft domains via a novel golgi-independent pathway. Identification of a novel membrane targeting domain and a role for palmitoylation." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48834-41, 2002 (PubMed).

Cabin, Shimazu, Murphy et al.: "Synaptic vesicle depletion correlates with attenuated synaptic responses to prolonged repetitive stimulation in mice lacking alpha-synuclein." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 20, pp. 8797-807, 2002 (PubMed).

Yamamori, Itakura, Sugaya et al.: "Differential expression of SNAP-25 family proteins in the mouse brain." in: The Journal of comparative neurology, Vol. 519, Issue 5, pp. 916-32, 2011 (PubMed).

Hosts (29), (6), (1)
Reactivities (30), (19), (15), (14), (13), (13), (1)
Applications (26), (9), (9), (4), (3), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (6), (2), (2), (1), (1), (1)
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