p62 Lck Ligand (AA 257-437) antibody

Antigen: p62 Lck Ligand

Binding Site: AA 257-437

Clonality: Monoclonal (3)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968203

Additional Information

Immunogen: Human p62 lck ligand

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 3

Description: P62 lck ligand (zeta-interacting protein (ZIP)) is a cytoplasmic protein that binds to the SH2 domain of lck (a T cell src tyrosine kinase) in the absence of a phosphotyrosine in either protein. The ubiquitously expressed p62 lck ligand contains a cysteine rich region that is similar to a zinc finger domain, a G protein binding region, a PEST sequence, and several phosphorylation sites. Deletion of the p62 lck ligand N-terminal domain has been reported to abrogate its binding to lck. However, mutation of the tyrosine did not have an effect. In addition, p62 lck ligand binds to the pseudosubstrate region of the PKCzeta catalytic domain. In turn, PKCzeta phosphorylates p62. p62 lck ligand binds to the dimerization region of PKCzeta, thereby inhibiting PKCzeta-PKCzeta interaction. This suggests that p62 lck ligand may compete with PKCzeta. However, it requires PKCzeta for proper subcellular localization. These data suggest that p62 lck ligand may be part of the protein bridge that links PKCzeta to the tyrosine kinases involved in signaling pathways. This antibody is routinely tested by western blot analysis.
Synonyms: Zeta Interacting Protein (ZIP)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 62 kDa

Comments:

Related Products: ABIN968551, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of p62 Ick ligand on a HCT-8 (human colorectal adenocarcinoma, ATCC CCL-244) cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- p62 Ick ligand antibody.

Immunofluorescence staining on FHs cells (normal human fetal lung fibroblasts, ATCC HTB-157).

Publications

Product: Joung, Strominger, Shin: "Molecular cloning of a phosphotyrosine-independent ligand of the p56lck SH2 domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 12, pp. 5991-5, 1996 (PubMed).

Puls, Schmidt, Grawe et al.: "Interaction of protein kinase C zeta with ZIP, a novel protein kinase C-binding protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 12, pp. 6191-6, 1997 (PubMed).

Wooten, Seibenhener, Mamidipudi et al.: "The atypical protein kinase C-interacting protein p62 is a scaffold for NF-kappaB activation by nerve growth factor." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 7709-12, 2001 (PubMed).

Cariou, Perdereau, Cailliau et al.: "The adapter protein ZIP binds Grb14 and regulates its inhibitory action on insulin signaling by recruiting protein kinase Czeta." in: Molecular and cellular biology, Vol. 22, Issue 20, pp. 6959-70, 2002 (PubMed).