You are viewing an incomplete version of our website. Please click to reload the website as full version.

RAB4A, Member RAS Oncogene Family (RAB4A) (AA 97-213) antibody

Details for Product No. ABIN968232, Supplier: Login to see
Antigen
  • rab4a
  • MGC52945
  • fc93b03
  • wu:fc93b03
  • RAB4A
  • HRES-1/RAB4
  • RAB4
  • AI848268
  • Rab4
Epitope
AA 97-213
23
7
4
3
3
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
74
41
39
4
1
Host
Mouse
55
18
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
56
29
26
22
18
11
8
5
2
1
1
Supplier
Login to see
Supplier Product No.
Login to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Immunogen Human Rab4 aa. 97-213
Clone 7-Rab4
Isotype IgG1
Cross-Reactivity Rat (Rattus), Dog (Canine), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Rab4 (RAB4A Antibody Abstract)
Background The Rab proteins are small GTP-binding molecules that are localized to specific intracellular vesicles and organelles and are important for vesicular trafficking. The Rab proteins cycle between active GTP-bound and inactive GDP-bound states. Rab4 is associated with early endosomes and recycling vesicles. It is involved in the recycling of receptors to the plasma membrane and regulates membrane recycling from early endosomes. Rab4 has been reported to be membrane-bound during interphase. However, it can be found as a cytosolic protein during mitosis. It is thought that Rab4 is phosphorylated by cdc2/cyclin B during mitosis and that Ser196 on Rab4 is the recognition site of cdc2. Mutation of Ser196 results in Rab4 membrane association during mitosis in an unphosphorylated form. Together this data indicates that Rab4 is phosphorylated by cdc2 on Ser196 which releases it into the cytosol during mitosis. In addition, Rab4 may play a role in insulin-induced glucose transport into rat adipocytes. Rab4 has also been reported to be associated with vesicles that contain GLUT4, a glucose transporter isoform. This antibody is routinely tested by western blot analysis.
Molecular Weight 25 kDa
Research Area Cancer, Organelles, Signaling
Pathways
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-RAB4A, Member RAS Oncogene Family (RAB4A) (AA 97-213) antibody (ABIN968232) Western blot analysis of Rab4 on a rat cerebrum lysate. Lane 1: 1:1000, lane 2: 1:200...
Immunofluorescence (IF) image for anti-RAB4A, Member RAS Oncogene Family (RAB4A) (AA 97-213) antibody (ABIN968232) Immunofluorescence staining of L6 cells (rat skeletal muscle myoblasts, ATCC CRL-1458).
Product cited in: Steiner, Sarria, Glauser et al.: "Modulation of receptor cycling by neuron-enriched endosomal protein of 21 kD." in: The Journal of cell biology, Vol. 157, Issue 7, pp. 1197-209, 2002 (PubMed).

Sans, Racca, Petralia et al.: "Synapse-associated protein 97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 19, pp. 7506-16, 2001 (PubMed).

Cormont, Bortoluzzi, Gautier et al.: "Potential role of Rab4 in the regulation of subcellular localization of Glut4 in adipocytes." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6879-86, 1997 (PubMed).

Zahraoui, Touchot, Chardin et al.: "The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion." in: The Journal of biological chemistry, Vol. 264, Issue 21, pp. 12394-401, 1989 (PubMed).

Background publications Huang, Imamura, Olefsky: "Insulin can regulate GLUT4 internalization by signaling to Rab5 and the motor protein dynein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 23, pp. 13084-9, 2001 (PubMed).