SSB (AA 179-289) antibody

Antigen: SSB

Binding Site: AA 179-289

Clonality: Monoclonal (44)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968242

Additional Information

Alternative name: La Protein

Immunogen: Human La Protein

Cross-Reactivity: Human, Dog (Canine), Rabbit, Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 44

Description: La was first found to bind autoantibodies from individuals with Sjogren's syndrome and systemic lupus erythematosus (SLE). La is a transcription factor for RNA polymerase III (Pol III). It participates in the termination of Pol III-mediated transcription and the recycling of the transcriptional machinery. La associates with the 3' oligo U portion of transcripts generated by Pol III. In some cases, this association protects the message from 3' processing. The association of La and mRNA is mediated by the presence of an N-terminal ribonucleoprotein (RNP) consensus sequence in La. The La protein also binds several small RNAs, such as 7S RNA, 5S RNA, and tRNA, and is thought to be involved in their biogenesis. It is known that histone mRNA is stabilized throughout S Phase and is degraded only at the end of S phase. La protein participates in the stabilization of histone mRNA which results in increased production of histone protein. These data suggest that a major function of La is to protect vital nascent transcripts from premature degradation.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 47 kDa

Comments:

Related Products: ABIN968551, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of La Protein on HCT-8 lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-La Protein antibody.

Immunofluorescent staining of HeLa cells.

Publications

Product: Chambers, Kenan, Martin et al.: "Genomic structure and amino acid sequence domains of the human La autoantigen." in: The Journal of biological chemistry, Vol. 263, Issue 34, pp. 18043-51, 1989 (PubMed).

McLaren, Caruccio, Ross: "Human La protein: a stabilizer of histone mRNA." in: Molecular and cellular biology, Vol. 17, Issue 6, pp. 3028-36, 1997 (PubMed).

Goodier, Fan, Maraia: "A carboxy-terminal basic region controls RNA polymerase III transcription factor activity of human La protein." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 5823-32, 1997 (PubMed).