Fatty Acid Synthase antibody (AA 9-202)

Catalog No. ABIN968278

This Mouse Monoclonal antibody specifically detects Fatty Acid Synthase in WB and IF. It exhibits reactivity toward Human, Mouse, Rat, Rabbit and Dog and has been mentioned in 3+ publications.

Quick Overview for Fatty Acid Synthase antibody (AA 9-202) (ABIN968278)

Target: Fatty Acid Synthase (FASN)

Reactivity: Human, Mouse, Rat, Rabbit, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fatty Acid Synthase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 23-Fatty Acid Synthase

Product Details anti-Fatty Acid Synthase Antibody

Binding Specificity: AA 9-202

Cross-Reactivity: Dog (Canine), Mouse (Murine), Rabbit, Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Fatty Acid Synthase aa. 9-202

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968587, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Fatty Acid Synthase antibody (ABIN968278)

Chirala, Huang, Jayakumar, Sakai, Wakil: "Animal fatty acid synthase: functional mapping and cloning and expression of the domain I constituent activities." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 11, pp. 5588-93, (1997) (PubMed).

Jayakumar, Tai, Huang, al-Feel, Hsu, Abu-Elheiga, Chirala, Wakil: "Human fatty acid synthase: properties and molecular cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 19, pp. 8695-9, (1995) (PubMed).

Smith: "The animal fatty acid synthase: one gene, one polypeptide, seven enzymes." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 8, Issue 15, pp. 1248-59, (1995) (PubMed).

Target Details for Fatty Acid Synthase

Target: Fatty Acid Synthase (FASN)

Alternative Name: Fatty Acid Synthase

Background: Fatty acid biosynthesis occurs in all living organisms and provides essential components of biological membranes as well as a form of energy storage. Animal fatty acid synthase (FAS) is a multifunctional enzyme that catalyzes the synthesis of long-chain fatty acids via sequential condensation of two-carbon units from malonyl-CoA, an intermediate derived from the carboxylation of Acetyl-CoA. FAS is a homodimer of a multifunctional subunit protein that contains seven distinct activities and a site for the prosthetic group 4'-phosphopantetheine (acyl carrier protein). These domains are oriented from N-terminus to C-terminus as follows: beta-keto-acyl synthase, acetyl and malonyl transacylases, enoyl reductase, ketoacyl reductase, acyl carrier protein, and thioesterase. Although all domains are found on each subunit, they are only active following the homodimerization of subunits in an antiparallel (head-to-tail) orientation. This juxtaposition and cooperation between domains forms two centers for acyl chain assembly. Alternative substrates and chain-terminating mechanisms allow for the production of a variety of fatty acids with different lengths and structures.

Molecular Weight: 265 kDa

Pathways: AMPK Signaling