Thymopoietin (TMPO) (AA 34-156) antibody

Antigen: Thymopoietin (TMPO)

Synonyms: TP, LAP2, CMD1T, LEMD4, PRO0868, MGC61508, LAP2beta

Binding Site: AA 34-156

Clonality: Monoclonal (27)

Host: Mouse

Reactivity: Rat (Rattus)

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968307

Additional Information

Alternative name: LAP2

Immunogen: Rat LAP2

Cross-Reactivity: Mouse (Murine), Human, Dog (Canine)

Format: Liquid

Isotype: IgG1

Clone: 27

Description: A specialized extension of the ER, the nuclear envelope (NE) forms the nuclear compartment boundary in eukaryotic cells. It contains numerous pore complexes and the nucleoplasmic side is linked to nuclear lamina. The nuclear lamina composes the structural framework for the NE and serves as a chromatin anchor site, thus, playing a major role in interphase nuclear organization. Many proteins are associated with lamina, particularly the LAPs (Lamina-Associated Polypeptides). LAP2 (also known as LAP2beta) is a hydrophilic protein with a single transmembrane segment near the C-terminus. Thus, it has been defined as a type II integral membrane protein with the majority of its structure exposed to the nucleoplasm. LAP2 binding to lamins contributes to the attachment of the nuclear lamina to the inner nuclear membrane. LAP2 also binds to chromatin, implying its role in chromosomal organization during mitosis. Mitotic phosphorylation of LAP2 regulates its binding to lamins and chromosomes during the disassembly and reassembly of mitosis. Thus, LAP2 is a nuclear protein that plays a role in the organization of the NE during cell cycle progression.
Synonyms: LAP2beta, Lamina-Associated Polypeptides

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 53 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Signaling, Cytoskeleton

Restrictions: For Research Use only

Images

Western blot analysis of LAP2 on a RSV-3T3 cell lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-LAP2 antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma, ATCC CCL-2.2).

anti-Thymopoietin (TMPO) (AA 34-156) antibody (Image 3)

Publications

Product: Furukawa, Panté, Aebi et al.: "Cloning of a cDNA for lamina-associated polypeptide 2 (LAP2) and identification of regions that specify targeting to the nuclear envelope." in: The EMBO journal, Vol. 14, Issue 8, pp. 1626-36, 1995 (PubMed).

Furukawa, Fritze, Gerace: "The major nuclear envelope targeting domain of LAP2 coincides with its lamin binding region but is distinct from its chromatin interaction domain." in: The Journal of biological chemistry, Vol. 273, Issue 7, pp. 4213-9, 1998 (PubMed).

Dechat, Gotzmann, Stockinger et al.: "Detergent-salt resistance of LAP2alpha in interphase nuclei and phosphorylation-dependent association with chromosomes early in nuclear assembly implies functions in nuclear structure dynamics." in: The EMBO journal, Vol. 17, Issue 16, pp. 4887-902, 1998 (PubMed).

Rusan, Tulu, Fagerstrom et al.: "Reorganization of the microtubule array in prophase/prometaphase requires cytoplasmic dynein-dependent microtubule transport." in: The Journal of cell biology, Vol. 158, Issue 6, pp. 997-1003, 2002 (PubMed).

Kimura, Ito, Watanabe et al.: "Mouse germ cell-less as an essential component for nuclear integrity." in: Molecular and cellular biology, Vol. 23, Issue 4, pp. 1304-15, 2003 (PubMed).