Dynactin p50 (AA 55-196) antibody

Antigen: Dynactin p50

Binding Site: AA 55-196

Clonality: Monoclonal (25)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968309

Additional Information

Immunogen: Human Dynactin p50

Cross-Reactivity: Rat (Rattus), Mouse (Murine), Insect

Format: Liquid

Isotype: IgG1

Clone: 25

Description: Dyneins are ubiquitous, multimeric proteins that are responsible for minus end-directed microtubule-based organelle transport. They are divided into two classes, cytosolic and axonemal. The activity of cytosolic dynein is regulated by the multiprotein dynactin complex with which it co-purifies. Dynactin is composed of at least nine polypeptides with p50 being the second most abundant subunit. The complex consists of an F-actin-like core filament of actin-related protein Arp1, a heterodimer that acts as a cap at one end, and a 62kDa subunit at the other end. p150[glued] is the largest subunit of the complex. The precise function of dynactin remains to be determined, but it may be recruited to prometaphase kinetochores to assist in alignment and spindle organization during mitosis. Additionally, dynactin localizes at the tail of cytosolic dynein and activates dynein-mediated vesicle movement. Therefore, dynactin may function to anchor or target dynein to the kinetochore during mitosis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 50 kDa

Comments:

Related Products: ABIN968537, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of Dynactin p50 on Jurkat cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Dynactin p50.

Immunofluorescent staining of WIDR cells.

Publications

Product: Echeverri, Paschal, Vaughan et al.: "Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis." in: The Journal of cell biology, Vol. 132, Issue 4, pp. 617-33, 1996 (PubMed).

Burkhardt, Echeverri, Nilsson et al.: "Overexpression of the dynamitin (p50) subunit of the dynactin complex disrupts dynein-dependent maintenance of membrane organelle distribution." in: The Journal of cell biology, Vol. 139, Issue 2, pp. 469-84, 1997 (PubMed).

Starr, Williams, Hays et al.: "ZW10 helps recruit dynactin and dynein to the kinetochore." in: The Journal of cell biology, Vol. 142, Issue 3, pp. 763-74, 1998 (PubMed).

Holleran, Ligon, Tokito et al.: "beta III spectrin binds to the Arp1 subunit of dynactin." in: The Journal of biological chemistry, Vol. 276, Issue 39, pp. 36598-605, 2001 (PubMed).

Kuo, Liu, Wei et al.: "Induction of human MDR1 gene expression by 2-acetylaminofluorene is mediated by effectors of the phosphoinositide 3-kinase pathway that activate NF-kappaB signaling." in: Oncogene, Vol. 21, Issue 13, pp. 1945-54, 2002 (PubMed).