RAD23 Homolog B (S. Cerevisiae) (RAD23B) (AA 73-193) antibody

Antigen: RAD23 Homolog B (S. Cerevisiae) (RAD23B)

Synonyms: P58, HR23B, HHR23B, p58, mHR23B, AV001138, 0610007D13Rik, MGC112630, MGC137555, RAD23B, MGC107846

Binding Site: AA 73-193

Clonality: Monoclonal (16)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968320

Additional Information

Alternative name: hHR23B

Immunogen: Human hHR23B

Cross-Reactivity: Dog (Canine)

Format: Liquid

Isotype: IgG1

Clone: 16

Description: Nucleotide excision repair (NER) is an essential element of DNA repair which eliminates unrelated DNA base lesions, including those induced by UV irradiation or chemicals. Although well defined in prokaryotes, eukaryotic NER is complex and involves at least two distinct subpathways. The transcription-coupled repair subpathway eliminates DNA lesions just prior to transcription. This is important for the removal of lesions for which the second pathway, global genome repair (i.e. covers the entire genome), is unable to repair. Individuals with defects in both pathways may suffer from xeroderma pigmentosum (XP), a condition with increased incidence of cancer in skin and internal organs. Mutations in seven genes result in human XP. However, one of these, XP-C, affects only the global genome repair pathway. The XP-C protein interacts with hHR23B, the human homolog of RAD23, an essential component of yeast NER. The XPC-hHR23B complex is required for excision of thymine dimers. In addition, hHR23B contains an N-terminal ubiquitin-like domain that allows for proteasome interaction. Thus, hHR23B may be an essential component of NER and mechanisms of DNA repair. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 58 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of hHR23B on a A431 cell lysate (Human epithelial carcinoma, ATCC CRL-1555). Lane 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-hHR23B antibody.

Immunofluorescence staining of WiDr cells (Human colorectal adenocarcinoma, ATCC CCL-218).

Publications

Product: Masutani, Sugasawa, Yanagisawa et al.: "Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homologue of yeast RAD23." in: The EMBO journal, Vol. 13, Issue 8, pp. 1831-43, 1994 (PubMed).

Reardon, Mu, Sancar: "Overproduction, purification, and characterization of the XPC subunit of the human DNA repair excision nuclease." in: The Journal of biological chemistry, Vol. 271, Issue 32, pp. 19451-6, 1996 (PubMed).

Sugasawa, Masutani, Uchida et al.: "HHR23B, a human Rad23 homolog, stimulates XPC protein in nucleotide excision repair in vitro." in: Molecular and cellular biology, Vol. 16, Issue 9, pp. 4852-61, 1996 (PubMed).

Wakasugi, Sancar: "Assembly, subunit composition, and footprint of human DNA repair excision nuclease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 12, pp. 6669-74, 1998 (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, 2002 (PubMed).