PARP (AA 22-219) antibody

Antigen: PARP

Binding Site: AA 22-219

Clonality: Monoclonal (42)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968332

Additional Information

Immunogen: Human PARP

Cross-Reactivity: Dog (Canine), Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 42

Description: Poly(ADP-ribose) polymerase (PARP) is a constitutively expressed, abundant nuclear protein. It has been referred to as a molecular nick sensor due to its recognition of, and catalytic activation by, single or double strand DNA breaks. The most critical ad extensively studied role of PARP is its participation in DNA base excision repair. Following binding to damaged DNA, PARP uses NAD+ to synthesize branched polymers of poly(ADP-ribose) on nuclear target proteins, including itself. Such modification of PARP increases its negative charge and results in loss of interactionwith DNA due to electrostatic repulsion. This opens the damaged DNA to DNA repair proteins. The poly(ADP-ribose) molecule is quickly degraded by poly (ADP-ribose) glycohydrolase that is found in association with PARP. PARP contains N-terminal DNA-binding domain (DBD), a central automodification domain that accepts poly (ADP-ribose), and a C-terminal catalytic domain. PARP is one of the earliest proteins targeted by caspase-3 during apoptosis. Although this protein is central to DNA repair, it has additional DNA-related functions that remain to be investigated. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 113 kDa

Comments:

Related Products: ABIN968537, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA, Enzymes, Metabolism

Restrictions: For Research Use only

Images

Western blot analysis of PARP on Jurkat cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-PARP antibody.

Immunofluorescent staining on BC3H1 cells

Publications

Product: Cherney, McBride, Chen et al.: "cDNA sequence, protein structure, and chromosomal location of the human gene for poly(ADP-ribose) polymerase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 23, pp. 8370-4, 1988 (PubMed).

Duriez, Shah: "Cleavage of poly(ADP-ribose) polymerase: a sensitive parameter to study cell death." in: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 75, Issue 4, pp. 337-49, 1998 (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, 2002 (PubMed).

Liao, Chien, Shenoy et al.: "Inhibition of constitutively active forms of mutant kit by multitargeted indolinone tyrosine kinase inhibitors." in: Blood, Vol. 100, Issue 2, pp. 585-93, 2002 (PubMed).

Jaiswal, Marlow, Gupta et al.: "Beta-catenin-mediated transactivation and cell-cell adhesion pathways are important in curcumin (diferuylmethane)-induced growth arrest and apoptosis in colon cancer cells." in: Oncogene, Vol. 21, Issue 55, pp. 8414-27, 2002 (PubMed).