LAMP1 antibody (AA 25-224)

Catalog No. ABIN968337

This anti-LAMP1 antibody is a Mouse Monoclonal antibody detecting LAMP1 in WB, IF and IP. Suitable for Human. This Primary Antibody has been cited in 5+ publications.

Quick Overview for LAMP1 antibody (AA 25-224) (ABIN968337)

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAMP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 25-Lamp

Product Details anti-LAMP1 Antibody

Binding Specificity: AA 25-224

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Lamp-1 aa. 25-224

Isotype: IgG2b

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-LAMP1 antibody (ABIN968337)

Alto, Soderling, Scott: "Rab32 is an A-kinase anchoring protein and participates in mitochondrial dynamics." in: The Journal of cell biology, Vol. 158, Issue 4, pp. 659-68, (2002) (PubMed).

Hocking, Kowalski: "A cryptic fragment from fibronectin's III1 module localizes to lipid rafts and stimulates cell growth and contractility." in: The Journal of cell biology, Vol. 158, Issue 1, pp. 175-84, (2002) (PubMed).

Spoerl, Stumpf, Noegel, Hasse: "Oligomerization, F-actin interaction, and membrane association of the ubiquitous mammalian coronin 3 are mediated by its carboxyl terminus." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48858-67, (2002) (PubMed).

Fukuda: "Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficking." in: The Journal of biological chemistry, Vol. 266, Issue 32, pp. 21327-30, (1991) (PubMed).

Fukuda, Viitala, Matteson, Carlsson: "Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Comparison of their deduced amino acid sequences." in: The Journal of biological chemistry, Vol. 263, Issue 35, pp. 18920-8, (1989) (PubMed).

Target Details for LAMP1

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Alternative Name: Lamp-1

Background: Lysosomes are digestive compartments in mammalian cells that are responsible for the degradation of numerous substances including foreign materials. The lysosomal membrane is of interest due to its role in maintaining the acidic intralysosomal sac, its resistance to degradation, and its ability to fuse with other membranous organelles. Lamp-1 and lamp-2 (lysosomal-associated membrane proteins -1 and -2) were identified as lysosomal membrane proteins that act as carriers for poly-N-glycans, primarily N-acetyllactosamines. They consist of two heavily glycosylated amino terminal domains, a single trans-membrane domain, and a short cytoplasmic tail. A tyrosine located at the fourth residue from the end of the cytoplasmic tail, a large hydrophobic amino acid as the last residue, and a glycine residue amino terminal to the tyrosine residue targets these proteins from the TGN to the lysosome. The role of the Lamp molecules may be a carrier for poly-N-acetyllactosamines and/or to protect lysosomal membranes from the soluble hydrolases. This antibody is routinely tested by western blot analysis.

Molecular Weight: 110 kDa

Pathways: Autophagy